FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_plasmid_mix.3510000003f856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_plasmid_mix.3510000003f856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320466
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG244507.629514519481005No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT143004.462251845749627TruSeq Adapter, Index 6 (97% over 36bp)
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG64702.0189349260139924No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC23170.7230096172448871No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT11330.35354764624016277No Hit
GCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGGT6380.19908508234882952No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAACCTCGTAT4640.14478915079914875TruSeq Adapter, Index 6 (97% over 36bp)
ACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCT4480.13979642146124707No Hit
CTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATAC3700.11545686593897636No Hit
GGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCC3490.10890390868298042No Hit
AATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCC3260.10172686025974674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT26650.0285.03758
GGGCGTC26650.0283.930579
CGCGCTC2800.0252.857134
GCCCGTG1250.0247.799995
GGCGGTG7850.0242.388538
GAGGGCG31750.0239.251977
CGCCCGT1350.0229.444464
GGGCGGT8450.0225.177527
CGGGGGC8800.0216.221594
GGAGGGC36350.0209.786776
CGTGCCA1500.0206.499988
GGCCGCG3500.0202.285721
GGGGGCG10000.0193.224995
GGGAGGG42700.0179.279865
CCGCGCT4150.0170.60243
CGGCCAT351.0054537E-6168.57143295
CCCGTGC1950.0158.846156
GACTGTC200.0067354445147.5295
GCCGGGA56850.0133.878632
CGGGAGG58450.0129.96154
Produced by FastQC (version 0.11.2)