FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b9.3510000003f515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b9.3510000003f515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224101
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA36871.6452403157504873No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG36281.6189129008795142No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC21090.9410935247946238No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA18860.8415848211297585No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC18170.8107951325518404No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT17930.8000856756551733No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT17320.7728658060428111No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA16700.7451997090597543No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT15730.7019156541023913No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14630.6528306433260004No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT13690.6108852704807207No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT12500.5577842133680796No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12330.5501983480662738No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT10260.45782928233251974No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA9710.43328677694432427No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT8930.398481042030156No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT8760.39089517672835017No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT8530.3806319472023775No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC7300.3257459806069585No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG6890.30745065840848546No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG6010.26818264978737266No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5620.2507797823302886No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT5430.2423014622870938No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5250.2342693696145934No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA4440.19812495258834184No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4230.18875417780375814No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4230.18875417780375814No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC3770.1682277187518128No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA3630.16198053556209033No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT3490.1557333523723678No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT3460.15439467026028442No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA3210.14323898599292284No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3140.1401153943980616No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA3140.1401153943980616No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2650.11825025323403288No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2620.11691157112194948No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2570.11468043426847715No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2300.10263229525972664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGG108.460489E-4295.0295
CCGGGTT24350.0294.394261
CGGGTTA24700.0290.819822
GGGGGGA18850.0269.177731
TATTAGT27550.0261.80587
TTATTAG28550.0252.635746
GGAGCAG20100.0250.236315
ATTAGTA28950.0249.145088
GTTATTA29050.0248.287455
GGGTTAT29450.0244.915123
CAGAAGC20450.0244.511029
TTAGTAG29700.0242.853539
GAGCAGA20800.0241.814916
GGTTATT31900.0226.105014
GGGAGCA22350.0225.70474
GGGGAGC24300.0207.592593
GCAGAAG25400.0197.440958
GGCGTGG150.0028481723196.66667295
GGGGGAG25800.0195.523252
AGCAGAA27500.0182.363637
Produced by FastQC (version 0.11.2)