FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b8.3510000003f4b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b8.3510000003f4b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196576
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA36621.8628927234250365No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA30741.5637717727494709No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT28481.4488035161972979No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC28061.427437734006186No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA27731.4106503337131695No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA26491.3475704053394106No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT20391.0372578544685007No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT19300.9818085625915676No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG18450.9385682891095556No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT14420.7335585218948396No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11860.6033289923490152No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT9890.5031132996907048No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT9240.47004720820446033No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC9100.4629252808074231No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT8310.42273726192414124No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT7890.40137147973302945No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA7820.3978105160345108No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7600.3866189158391665No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG7480.380514406641706No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7380.37542731564382226No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT6750.3433786423571545No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT6150.3128560963698519No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA5570.283350968582126No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5450.27724645938466547No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT3820.19432687611916002No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3670.18669623962233436No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA3580.18211785772423897No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3520.1790656031255087No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG3310.16838271202995278No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA3290.16736529383037602No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2980.15159531173693636No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTAT2380.12107276574963373No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT2380.12107276574963373No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2060.10479407455640567No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATCTATTTTGACAAGA2020.10275923815725216No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2010.10225052905746379No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAG108.4596983E-4295.07
CCGGGTT23450.0291.2261
CGGGTTA23750.0287.547362
GGGGGGA19150.0282.676241
ATTAGTA25250.0269.297038
TATTAGT25250.0269.297037
GTTATTA25850.0263.617035
TTATTAG26800.0254.272386
TTAGTAG27450.0247.714029
GGGGAGC22900.0236.386443
GGAGCAG22800.0236.12945
GAGCAGA23300.0231.062236
GGGGGAG23950.0226.638822
CAGAAGC23850.0225.733759
GGGTTAT30550.0223.543373
GGTTATT31800.0214.756294
CAGCGAA353.965397E-9210.71428295
GGGAGCA26200.0205.486634
GGAGCGA150.0028479071196.666675
GGGAGCG150.0028479071196.666674
Produced by FastQC (version 0.11.2)