FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b7.3510000003f4aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b7.3510000003f4aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179135
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAGCAATCAAGTTCAG33561.8734474000055825No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA33371.8628408742010216No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC18271.0199011918385574No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA17470.9752421358193542No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC17010.9495631786083122No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14490.8088871521478214No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA13780.7692522399307785No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11180.6241103078683674No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9960.5560052474390823No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA9240.5158120970217992No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTTGTTCTTTATAT9050.5052055712172384No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT8170.4560806095961147No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTT7700.4298434141848327No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT6330.3533647807519468No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC6190.3455494459485863No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTTGTTCTTTATAT5980.33382644374354536No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG5890.328802299941385No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5460.3047980573310632No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4110.22943590029865743No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4080.2277611856979373No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAGCAATCAAGTTCAG3960.22106232729505682No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3880.21659642169313648No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTTCATTTTAATCATTTGTT3870.21603818349289644No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3670.20487341948809557No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT3510.19594160828425491No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTT3110.1736120802746532No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT3100.17305384207441316No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC3080.17193736567393306No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2820.15742317246769194No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2590.144583693862171No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG2510.14011778826025065No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2360.13174421525665No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2350.13118597705640997No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTCATT2230.12448711865352946No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2130.11890473665112905No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2080.11611354564992882No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACG108.459073E-4295.0295
TGACTTA108.459073E-4295.0295
CCGGGTT18000.0291.722231
CGGGTTA18400.0285.380432
GGGGGGA14300.0270.244751
GGGTTAT20750.0253.060243
TATTAGT20650.0252.142877
ATTAGTA20700.0251.533818
GGAGCAG15450.0250.129445
GGGGAGC15850.0243.817033
TTATTAG21650.0241.177816
GTTATTA21900.0238.424675
GAGCAGA16200.0237.638896
TTAGTAG22750.0228.219779
GGGAGCA17000.0227.323534
GGTTATT23150.0226.187914
CAGAAGC19450.0197.172239
GGGGGAG20400.0190.882342
GCAGAAG21450.0179.475528
AGCAGAA25600.0149.804697
Produced by FastQC (version 0.11.2)