FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b57.3510000003f82f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b57.3510000003f82f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199689
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA34241.7146663061059948No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC33611.6831172473195821No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA27401.3721336678535123No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA24481.2259062842720432No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT23471.1753276344716033No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT21951.099209270415496No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG19740.9885371753076032No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT17850.8938899989483646No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA15680.7852210186840536No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT15630.7827171251295765No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA15280.765189870248236No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT14790.7406517134143593No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT12180.6099484698706489No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG11590.5804025259278178No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC10630.5323277696818552No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT10400.5208098593312601No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT10330.517304408354992No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10170.509291948980665No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT9820.49176469409932444No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA9740.4877584644121609No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA9000.45070083980589815No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7650.38309571383501345No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT7620.38159337770232715No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT7440.37257936090620913No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG7430.3720785821953137No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG6930.3470396466505416No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT5930.29696177556099734No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5690.2849430864995067No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4670.23386365798817163No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4360.2183395179504129No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT4260.21333173084145846No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA4180.20932550115429494No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA4140.20732238631071317No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT4090.20481849275623595No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT3450.17276865525892765No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT3300.165256974595496No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG3280.16425541717370512No Hit
CCGGGTTATTAGTAGAAACAACAGCACTGGGTCCATGGGGCTCCGCTTCC3220.16125074490833247No Hit
CCGGGTTATTAGTAGAAACAACAGCTATTACGCATTGCGCAGCTTTAACT3080.15423984295579626No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3070.15373906424490083No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2890.14472504744878287No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2820.14121959647251475No Hit
CCGGGTTATTAGTAGAAACAACAGCGATCCGAAATCCCCACTGCATAGAC2710.1357110306526649No Hit
GGGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC2590.12970168612191957No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2550.1276985712783378No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT2370.11868455448221986No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2140.10716664413162467No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA108.4597984E-4295.02
CCGGGTT34350.0293.282381
CGGGTTA34650.0290.743132
GGGGGGA21950.0284.92031
TATTAGT36400.0277.980777
ATTAGTA36500.0277.219188
GTTATTA37000.0272.277045
TTATTAG37900.0265.811346
TTAGTAG40650.0248.91769
GGGTTAT40950.0245.653233
GGGGAGC25700.0243.920243
GGTTATT41650.0241.878754
CAGAAGC27150.0230.893199
GGGGGAG27600.0227.128622
GGAGCAG28500.0219.956135
GGGAGCA28850.0217.287694
GAGCAGA29300.0213.950536
GCAGAAG30300.0206.889438
AGCAGAA31450.0199.324337
GCACGCG150.0028479407196.66667295
Produced by FastQC (version 0.11.2)