FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b56.3510000003f812.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b56.3510000003f812.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172348
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA54093.1384176201638545No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG33571.947803281732309No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC25331.4697008378397196No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA23761.3786060760786316No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA22341.2962146355049087No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA18531.0751502773458352No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA17351.0066841506718964No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14630.8488639264743426No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10190.5912456193283357No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT10160.5895049550908626No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT9290.5390256922041451No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA8590.49841019332977465No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT8440.4897068721424095No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8290.48100355095504443No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA7830.45431336598045813No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGGTTCTTGTTTCTTTTTAT7200.4177594169935247No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG7020.4073154315686866No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA5860.34000974771972986No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG5500.3191217768700536No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA5020.2912711490704853No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT4950.2872095991830483No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT4510.26167985703344393No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT4490.260519414208462No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGGTTCTTGTTTCTTTTTAT4160.24137210759625877No Hit
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA4050.23498967205885765No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT4040.23440945064636667No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA3580.20771926567178037No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA3280.19031262329705015No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT3020.17522686657228398No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG2910.16884443103488292No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA2890.16768398820990088No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2870.1665235453849189No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA2780.16130155267249982No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA2730.1584004456100448No Hit
GGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAA2640.15317845289762574No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2620.15201801007264373No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT2580.14969712442267968No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2110.12242671803560237TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2090.12126627521062038No Hit
GGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGAA2070.12010583238563836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT21000.0293.595251
GGGGGGA17450.0289.928381
CGGGTTA21550.0286.10212
GTTATTA21800.0282.82115
TATTAGT23300.0263.347637
GGGTTAT23700.0260.147673
TTATTAG23800.0258.434886
ATTAGTA23800.0257.815128
GGGGAGC19850.0254.130983
TTAGTAG24750.0247.91929
GGAGCAG20800.0241.814915
GAGCAGA21050.0239.64376
CAGAAGC21250.0235.305889
GGTTATT27250.0226.256884
GGGGGAG22500.0225.511112
GGGAGCA22600.0222.555314
GCAGAAG23350.0214.775168
AGCAGAA24350.0205.954837
CAAGGTT150.0028476035196.66667295
TCTCGTG150.0028476035196.66667295
Produced by FastQC (version 0.11.2)