FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b55.3510000003f805.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b55.3510000003f805.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198344
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTTTTGTAAT33291.6783971282216754No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA32381.6325172427701367No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG26521.3370709474448432No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC23001.1596015004235067No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA20741.045658048642762No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC16320.8228128907352882No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT15980.8056709555116363No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA14450.728532247005203No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14160.7139111846085588No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTTTTGTAAT11800.5949259871737991No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT10780.5435001815028435No Hit
GGGGGGAGCAGAAGCGGTGCATTTGATTTGTCATAATGGATACTTTTATT10310.5198039769289718No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT10190.5137538821441536No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT10040.5061912636631307No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG10010.5046787399669261No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTGTAT9470.47745331343524383No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC8280.4174565401524624No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7590.38266849513975715No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC7140.3599806396966886No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTGTAT6200.31258823054894525No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT6180.31157988141814225No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT6140.3095631831565361No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG5320.2682208687936111No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT5200.2621707740087928No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5000.2520872827007623No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4770.24049126769652723No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA4740.23897874400032265No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3470.17494857419432905No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3280.16536925745170009No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3130.1578066389706772No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2860.14419392570483605No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2820.14217722744322994No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT2680.1351187835276086No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2600.1310853870043964No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG2530.12755616504658573No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2530.12755616504658573No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT2330.11747267373855524No Hit
GGGGGGAGCAGAAGCGGAGCATTTGATTTGTCATAATGGATACTTTTATT2220.11192675351913847No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2070.1043641350381156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCA108.4597553E-4295.0295
CCGGGTT24000.0293.156251
CGGGTTA24400.0288.35042
GGGGGGA18500.0265.51
ATTAGTA27000.0259.490758
TATTAGT27050.0259.011087
GGGTTAT27250.0258.192663
TTATTAG27200.0257.040446
GTTATTA28000.0250.223225
GGGGAGC20100.0244.365693
TTAGTAG29050.0241.178999
CAGAAGC20500.0236.719519
GGAGCAG20900.0234.306215
GGTTATT30000.0234.033334
GAGCAGA21000.0232.48816
GGGAGCA23100.0211.991354
CTCCTGA353.965397E-9210.71428295
GGGGGAG24800.0198.054432
GAATCGA150.002847926196.66667295
GCAGAAG26200.0185.782448
Produced by FastQC (version 0.11.2)