FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b54.3510000003f7f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b54.3510000003f7f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281276
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA78732.7990301341031585No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC76592.7229482785591377No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA72922.5924714515280365No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT66642.3692032025483867TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT50271.787212559905573No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA21700.7714842361239495No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA20480.7281104680100684No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGA19150.680825950312149No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG16430.5841237787795617No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT16390.5827016880217296No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG16280.5787909384376911No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA14980.5325729888081457No Hit
GGGGGGAGGGAAAAATCTGTGTACTTGTACTGCCGAGTGAATGGCACAAA14360.5105305820617472No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA14310.508752968614457No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG13760.48919922069426475No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT13190.46893442739515634No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA13120.46644576856895004No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT12770.4540024744379186No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA11050.3928525718511355No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT10570.37578748275714957No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG9540.3391686457429713No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT8770.3117933986547021No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT7970.28335158349805883No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT6990.24851035993117082No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG6350.2257569078058562No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6100.21686884056940514No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA5790.20584763719620586No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAATTACTAGA5400.19198225230734225No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT5160.18344970776034927No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATGAGA5050.1795389581763108No Hit
GAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATGAG4950.1759837312817304No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4900.1742061178344402No Hit
AGAAGCGGTGCCATGGATGAACTCCACAACGAATTACTAGAACTAGATGA4590.16318491446124092No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA4580.16282939177178288No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3980.1414980304043004No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA3890.13829832619917803No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAAT3760.1336765312362235No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA3600.12798816820489484No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT3500.12443294131031443No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT3270.11625591945277948No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTG3180.11305621524765709No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT3010.10701232952687041No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2960.1052347160795802No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA2830.10061292111662568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGT157.234561E-6295.0295
TCCCGGC108.461634E-4295.0295
CCGGGTT32750.0292.748081
GGGGGGA34500.0289.869571
CGGGTTA33550.0285.767522
GGGTTAT34500.0277.898563
ATTAGTA36000.0266.72928
TATTAGT36100.0266.39897
GTTATTA37100.0258.02565
TTAGTAG37650.0255.039849
GGGGAGC38400.0250.058613
TTATTAG39500.0242.721516
GGGGGAG41450.0241.978292
GGTTATT39750.0241.194964
GGAGCAG40050.0239.388285
GAGCAGA40800.0235.349276
CAGAAGC41650.0229.837929
AGCAGAA42550.0225.66987
GCAGAAG44650.0214.725658
GGGAGCA50350.0191.002994
Produced by FastQC (version 0.11.2)