FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b53.3510000003f7ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b53.3510000003f7ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217387
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA37681.7333143196235283No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA29851.373127187918321No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC28261.2999857397176464No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG27451.2627250019550387No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA23861.097581732118296No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA21240.9770593457750464No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC17170.7898356387456472No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15120.6955337715686771No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT13430.6177922322861993No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT12920.5943317677690018No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT11760.5409707112200821No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11100.5106101100801795No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA8680.39928790590053687No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT8270.38042753246514277No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG7330.33718667629619065No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT6860.3155662482117146No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA6430.29578585655995987No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT6130.2819855833145496No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5750.2645052372036967No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG5630.2589851279055326No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT5170.23782470892923682No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT4290.1973439074093667No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3950.1817035977312351No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3900.17940355219033338No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3670.16882334270218552No Hit
CCGGGTTATTAGTAGTAACAAGAGCAGTACTCAAACAAATGAGAGACCAA3360.1545630603485949No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3350.15410305124041457No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3350.15410305124041457No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC3340.1536430421322342No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT3090.14214281442772567No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2790.12834254118231542No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2550.1173023225859872No Hit
CCGGGTTATTAGTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGG2490.11454226793690515No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTCTGGGTAACAGTGAACACGGGGA2480.1140822588287248No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT2400.11040218596328208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG108.4603135E-4295.0295
CCGGGTT24650.0289.614621
CGGGTTA25200.0282.70832
GGGGGGA20400.0269.693631
ATTAGTA27500.0258.527258
TATTAGT27500.0258.527257
TTATTAG27950.0254.364946
GAGCAGA22200.0241.846856
GGGGAGC23150.0236.38233
GGGTTAT30250.0235.512393
GGAGCAG23050.0234.208255
TTAGTAG31100.0228.601279
CAGAAGC23850.0225.11539
GTTATTA31600.0224.984185
GGGAGCA24700.0219.159934
GGTTATT33400.0213.742524
GGGGGAG27400.0200.255482
AGCGAAG150.0028481137196.666677
GCAGAAG28650.0188.429328
AGCAGAA30550.0176.71037
Produced by FastQC (version 0.11.2)