FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b506.3510000003f849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b506.3510000003f849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173258
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA32261.8619630839557193No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTCAAATTCACAAGCAC24961.4406261182744808No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA21541.2432326357224486No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT19721.1381869812649343No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG17451.007168500155837No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA16130.9309815419778597No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC12280.7087695806254257No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT11800.6810652321970703No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10400.600260882614367No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA10190.5881402301769615No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT10020.5783282734419191No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT9160.5286913158411155No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT8940.5159934894781193No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA8310.47963153216590293No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT8180.4721282711332233No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT7930.4576989229934549No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA7790.44961848803518456No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG7460.4305717484906902No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA7250.4184510960532847No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG5740.3312978332890833No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5440.3139826155213612No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT4690.2706945711020559No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA4560.26319131006937635No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTG4260.24587609230165416No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG3550.2048967435847118No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTATCATAATGGATACTTTTATT3390.19566196077526002No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA3310.19104456937053413No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTATGATGACCTCGATTATTAAAT2940.16968913412367684No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT2830.1633402209421787No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2630.15179674243036395No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2320.13390435073705112No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2230.12870978540673447No Hit
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA2080.12005217652287341No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2080.12005217652287341No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC1970.11370326334137529No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT1940.11197174156460307No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1910.11024021978783087No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA1910.11024021978783087No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT1820.10504565445751424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTT108.458834E-4295.0295
CCGGGTT21000.0293.595251
GGGGGGA14100.0290.81561
CGGGTTA21350.0288.782232
GTTATTA21750.0282.114965
GGGTTAT22600.0272.809723
TTATTAG23200.0264.482766
TATTAGT24250.0252.422687
ATTAGTA24400.0249.661888
GGGGGAG17950.0228.440122
GGGGAGC18050.0227.174513
GGTTATT27250.0225.715594
TTAGTAG27450.0222.459039
GGAGCAG19000.0216.592125
GAGCAGA19250.0213.0136
CAGAAGC19700.0207.398489
GGGAGCA20750.0198.32534
ATTAGTC150.0028476173196.666668
GCAGAAG23900.0172.186198
GTTTGTT351.0041349E-6168.57144295
Produced by FastQC (version 0.11.2)