FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b50.3510000003f7c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b50.3510000003f7c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191392
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA36671.9159630496572477No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC23361.220531683664939No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA23301.2173967563952515No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG20871.0904322019729142No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT17010.8887518809563618No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA16890.882482026416987No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA16510.8626274870423006No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT16420.8579250961377696No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT14350.7497701053335563No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT12700.6635596054171543No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12340.6447500417990302No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA12180.6363902357465306No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11970.6254179903026249No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11370.5940687176057515No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT9030.4718065540879452No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT8790.4592668450091958No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT8760.4576993813743521No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA8680.45351947834810236No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA8250.4310524995820097No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG7860.41067547232904195No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG7490.3913434208326367No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT7400.3866410299281057No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA5180.27064872094967396No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT5170.2701262330713927No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG4890.25549657247951846No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA4590.23982193613108177No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4570.23877696037451931No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTATCATAATGGATACTTTTATT3930.20533773616452097No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG3790.19802290586858384No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA3360.1755559271024912No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT3350.17503343922421No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC3130.16353870590202307No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT3050.15935880287577328No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA2830.14786406955358636No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2560.1337568968399933No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2330.12173967563952517TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA2240.11703728473499415No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGCGTTCATTG2160.11285738170874436No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2050.1071100150476509No Hit
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA2050.1071100150476509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT23450.0295.01
ACACGTT108.459524E-4295.0295
CGGGTTA23800.0290.661742
GGGGGGA19100.0290.36651
GTTATTA24050.0287.027045
GGGGGAG20800.0266.63462
TATTAGT25900.0266.52517
TTATTAG25950.0266.011576
ATTAGTA25950.0265.443158
GGGTTAT26200.0264.03633
TTAGTAG27600.0249.574269
GGGGAGC22500.0244.522223
GGAGCAG23750.0231.652635
GAGCAGA24050.0228.149696
CAGAAGC24000.0226.781239
GGTTATT31150.0222.078634
GGGAGCA25800.0213.246124
AGCAGAA26800.0203.638067
GCAGAAG27450.0198.816028
CCAGGTT2600.0147.5295
Produced by FastQC (version 0.11.2)