FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b49.3510000003f7b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b49.3510000003f7b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340153
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA303348.917751717609429No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA274568.071661869805647No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA61481.80742195423824No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA56991.675422530449533No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC51231.5060869667473167No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT47341.3917266641775905TruSeq Adapter, Index 1 (97% over 36bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA44131.2973573656560429No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT34421.0118975872622025No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG19520.5738594103241776No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAA18080.5315255193986236No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA17170.5047728522165026No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT16570.4871337309975217No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15250.44832766431576376No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA14550.42774868956028617No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10790.317210196588006No Hit
GGGGGGAGGGAAAAATCTGTGTACTTGTACTGCCGAGTGAATGGCACAAA8840.2598830526263181No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT7770.22842661978580225No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTTATATTTATTTTAATGAGA7200.21166945462777045No Hit
GGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAA6430.189032582396745No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA5600.16463179804382144No Hit
GGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAA5390.15845810561717816No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG5360.15757614955622912No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA5280.15522426672703166No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT5160.1516964424832355No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT5080.14934455965403803No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA4750.13964304298359856No Hit
GAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAG4750.13964304298359856No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4660.1369971748007514No Hit
CGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGAA4500.13229340914235654No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA4310.12670768742301258No Hit
GGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAAT4070.11965203893542023No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAATGAGA3830.11259639044782789No Hit
GAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAATA3790.11142044903322916No Hit
GGGGGGAGCAGAAGCAGAGGATGTAGATGGCCCAACTGAAATAGTATTAA3760.11053849297228012No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3750.11024450761863043No Hit
GCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAATAGA3740.10995052226498077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG305.456968E-12294.999971
GGGGGGC157.2358907E-6294.999972
CCGGGTT56050.0294.736881
GGGGGGA54000.0290.35651
CGGGTTA57250.0288.043672
GTTATTA57950.0285.327885
GGGTTAT58200.0283.595373
TATTAGT59450.0278.376777
ATTAGTA59850.0276.269848
TTAGTAG60450.0273.527749
GGTTATT60550.0272.58884
TTATTAG63250.0261.418986
GGGGAGC60200.0254.572253
GGGGGAG63450.0247.111912
GGAGCAG63150.0242.68015
CAGAAGC65300.0232.882849
GAGCAGA65850.0232.505696
AGCAGAA67050.0227.904547
GCAGAAG68950.0221.410458
GGGAGCA69300.0221.143574
Produced by FastQC (version 0.11.2)