FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b48.3510000003f7a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b48.3510000003f7a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147781
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA49753.366467949195093No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG46533.148577963337641No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA35962.433330401066443No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC28611.9359728246526955No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA27691.8737185429791379No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG25661.7363531171124842No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG25011.692369113756166No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT21981.4873359904182542No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA18341.2410255716228744No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT18101.2247853242297724No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT17941.2139584926343712No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT13230.8952436375447452No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA11420.7727651051217681No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAGACAATTAAGTTTAG11400.7714117511723428No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT10570.7152475622711987No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA10140.6861504523585576No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGCGTTCATTG9600.6496098957240782No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA9310.6299862634574134No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA8810.5961524147217843No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8370.5663786278344306No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAAT8290.56096521203673No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT7520.5088610849838612No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT5980.40465283087812376No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA5870.39720938415628526No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT4720.31939153206433846No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT4500.3045046386206617No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAAT4410.29841454584824845No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT4320.2923244530758352No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4110.278114236606871No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC3850.2605206352643438No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTG3820.2584906043402061No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA3520.2381902950988287No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT2910.19691299964136122No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT2680.18134942922297181No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATT2610.17661269039998376No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAGACAATTAAGTTTAG2540.1718759515769957No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTT2290.15495902720918114No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2210.1495456114114805No Hit
CCGGGTTATTAGTAGTAACAAGAGCTGACCTGGAGGTTTATGCTTTAACA1980.13398204099309113No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTC1980.13398204099309113No Hit
GGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTG1950.13195201006895338No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA1930.13059865611952823No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA1890.1278919482206779No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA1750.11841847057470174No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCT1680.11368173175171367No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA1650.11165170082757594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT27650.0292.866181
GGGGGGA22300.0291.03141
CGGGTTA27950.0289.722722
GTTATTA28450.0284.11255
TATTAGT28750.0281.660867
ATTAGTA28950.0279.715038
TTAGTAG29200.0277.32029
TTATTAG30600.0264.15036
TATGTTT4900.0255.86736295
GGGTTAT31750.0255.047243
GGGGGAG25800.0252.122122
GGTTATT32700.0247.63764
GGGGAGC26650.0243.527193
GGAGCAG29150.0222.13555
GAGCAGA29650.0218.389546
CAGAAGC30950.0209.693079
GGGAGCA30950.0209.216494
AGCAGAA31250.0207.679987
GCAGAAG31650.0205.05538
GTCAGTC150.0028471944196.66667295
Produced by FastQC (version 0.11.2)