FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b47.3510000003f79a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b47.3510000003f79a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149502
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA27481.8381025003009994No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG27181.818035879118674No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC26581.7779026367540234No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA21041.4073390322537491No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC14730.9852711000521732No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT13230.8849379941405466No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACATTTATTTTGACAAGA12820.8575136118580353No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT10610.7096895024815721No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT9350.6254096935158059No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT8960.5993230859787829No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT7880.5270832497224117No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7260.48561223261227277No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT6320.4227368195743201No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA6160.41203462161041327No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5650.3779213656004602No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5140.34380810959050717No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4700.3143770651897634No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4440.29698599349841476No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4030.2695616112159035No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT3750.2508327647790665No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3730.24949499003357814No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3640.24347500367888053No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT3590.24013056681515968No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT3330.22273949512381105No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTTAATTGTATTCATTG3100.20735508555069496No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2630.17591737903171864No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2340.1565196452221375No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACATTTATTTTGACAAGA2150.14381078513999812No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2090.13979746090353307No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1940.1297641503123704No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1850.1237441639576728No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTC1790.11973083972120775No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT1770.11839306497571939No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTTAATTGTATTCATTG1760.11772417760297521No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA1750.11705529023023103No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1750.11705529023023103TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT1710.11437974073925433No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGTA108.4576756E-4295.0295
CCGGGTT17950.0294.178251
CGGGTTA18000.0293.36112
ATTAGTA19650.0269.478368
TATTAGT19600.0269.413277
GGGTTAT20050.0263.366583
GGGGGGA13300.0262.838351
TTATTAG20950.0252.05256
GGTTATT21200.0249.775944
GTTATTA21450.0246.177175
GGGGAGC14300.0244.458043
TTAGTAG21950.0241.913449
GGAGCAG14900.0233.624165
CAGAAGC14950.0231.856199
GAGCAGA15300.0227.516336
GGGAGCA16950.0205.368734
GGGGGAG18550.0189.245292
GCAGAAG19750.0176.253168
AGCAGAA21850.0159.31357
TCTCGTT503.3373E-8147.5295
Produced by FastQC (version 0.11.2)