FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b44.3510000003f763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b44.3510000003f763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150775
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA38772.5713811971480682No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC37482.485823246559443No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA31922.117061847123197No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA21861.4498424805173271No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT20251.3430608522633063No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG17901.1871994694080583No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA14020.9298623777151384No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10100.6698723263140441No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA9630.6387000497429945No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA8610.5710495771845465No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT7990.5299287017078428No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT7030.4662576687116564No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT6670.4423810313380866No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6370.4224838335267783No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG5550.36809815950920244No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG5200.3448847620626762No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA4860.3223346045431935No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT4060.26927541037970487No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA4050.2686121704526612No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT3850.2553473719117891No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT3820.25335765213065825No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT3750.248714972641353No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT3620.2400928535897861No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG3110.2062676173105621No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2830.18769689935334105No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2810.18637041949925384No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT2720.1804012601558614No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA2520.1671364616149892No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA2520.1671364616149892No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA2310.15320842314707345No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT2290.15188194329298624No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2200.14591278394959376No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGCGTTCATTG2180.14458630409550655No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2100.13928038467915768No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG2050.13596418504393964No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA1950.12933178577350354No Hit
GAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAG1790.11871994694080583No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT1750.11606698723263141No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1710.11341402752445698TruSeq Adapter, Index 19 (97% over 38bp)
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT1600.10611838832697729No Hit
AGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGA1570.10412866854584645No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC1510.10014922898358482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA15450.0292.135931
CCGGGTT19750.0289.025331
CGGGTTA19950.0286.12782
GTTATTA20500.0279.170755
TATTAGT21300.0268.685427
ATTAGTA21350.0267.365368
GGGTTAT21650.0264.34183
TTAGTAG21900.0260.65079
TTATTAG22850.0249.814016
GGTTATT23200.0247.952594
GGAGCAG19050.0238.477685
GGGGGAG19550.0230.869572
GGGGAGC19650.0230.44533
GAGCAGA20100.0227.487586
CAGAAGC21750.0208.873559
AGCAGAA22750.0200.340657
GGGAGCA23050.0197.093284
AGTCGCC150.0028472517196.66667295
GCAGAAG23250.0195.397868
CCAGGTT1950.0151.28204295
Produced by FastQC (version 0.11.2)