FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b43.3510000003f757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b43.3510000003f757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282380
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTTACAAGCAC98583.4910404419576455No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA97643.4577519654366458No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT90593.208088391529145TruSeq Adapter, Index 7 (97% over 35bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA49751.7618103265103762No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT38721.3712019264820454No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT31051.0995821233798428No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA26050.9225157589064382No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT23540.8336284439407889No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT23480.8315036475671082No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA22090.7822791982435016No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT19930.7057865287909909No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA19630.6951625469225866No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA18420.6523124867200226No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG16870.5974219137332673No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTTAAAACTGAAGCAAATAGGCCAA14950.5294284297754799No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT14010.4961399532544798No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT12140.4299171329414264No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA12010.42531340746511787No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA11730.4153976910546073No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTACGTTTATTG10460.3704228344783625No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT9500.3364260924994688No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG7080.25072597209434094No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTACGTTTATTG6770.23974785749698987No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA6670.23620653020752178No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6260.22168708832070258No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC6190.21920815921807496No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT5850.2071676464338834No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT5710.2022097882286281No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTTAAAACTGAAGCAAATAGGCCAA4880.17281677172604293No Hit
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA4820.17069197535236205No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT4590.16254692258658546No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAGAATTG4200.1487357461576599No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTTAAAACTGAAGCAAATAGGCCAA3950.13988242793398967No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA3920.13882002974714924No Hit
CCGGGTTATTAGTAGAAACACGAGCTGGGAGATTCTTGCACAATTCCTCA3360.11898859692612793No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTTAAAACTGAAGCAAATAGGCCAAA3080.10907288051561724No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAGAATTG3080.10907288051561724No Hit
GGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGAA3030.10730221687088322No Hit
GGGGGGAGCAGAAGCGGAGCCATTAAGATGAATATAAATCCTTATTTTCT2960.10482328776825554No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTACCTCGTAT2930.10376088958141512TruSeq Adapter, Index 7 (97% over 35bp)
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCT2930.10376088958141512No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT2920.1034067568524683No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA2910.1030526241235215No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTACGTTTATTG2910.1030526241235215No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTACGTTTATTG2850.10092782774984063No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCTC2830.100219562291947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAC206.176742E-8295.0295
GGGGGGC108.461651E-4295.02
CCGGGTT36350.0291.348021
GGGGGGA35600.0287.12781
CGGGTTA37050.0285.843442
GTTATTA37550.0281.644475
TATTAGT40750.0259.889567
TTAGTAG41100.0257.67649
ATTAGTA42000.0252.154758
GGGTTAT42550.0248.895423
GGGGAGC42300.0239.905433
TTATTAG44400.0238.192576
GGGGGAG43450.0236.271582
GGAGCAG44450.0226.974125
GAGCAGA45850.0219.721916
GGTTATT48900.0216.876274
CAGAAGC47250.0212.275159
GCAGAAG48200.0208.09138
AGCAGAA48650.0206.16657
GGGAGCA55150.0183.739794
Produced by FastQC (version 0.11.2)