FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b42.3510000003f74a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b42.3510000003f74a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168344
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA35532.1105593308938837No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC22871.3585277764577293No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA22171.3169462529107068No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG22041.3092239699662596No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA16981.0086489568977808No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA13900.8256902532908805No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA13390.7953951432780496No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA12220.7258945967780259No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT12170.7229244879532386No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT10680.6344152449745758No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT10170.604120134961745No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9990.5934277431925106No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT9890.5874875255429359No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTATT9510.5649146984745521No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG8260.4906619778548686No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA7140.42413154017963217No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT6740.4003706695813335No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT6070.3605712113291831No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT6040.3587891460343107No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG5860.34809675426507625No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTATT5820.3457206672052464No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT5610.3332462101411396No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA5260.31245544836762823No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA5140.30532718718813856No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4600.2732500118804353No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT4290.25483533716675383No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA3550.21087772655990117No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG3350.19899729126075177No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA3300.19602718243596443No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2840.1687021812479209No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA2750.1633559853633037No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2470.1467233759444946No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG2370.14078315829491994No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA2340.1390010930000475No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTATCATAATGGATACTTTTATT2280.1354369624103027No Hit
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA2230.13246685358551535No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2110.12533859240602577No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT2060.12236848358123842No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC1820.10811196122225918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT19800.0292.765141
GGGGGGA17750.0290.01411
CGGGTTA20000.0289.83752
GTTATTA20150.0287.67995
GGGTTAT21850.0265.29753
TTATTAG21950.0264.088846
TATTAGT22400.0260.100437
ATTAGTA22600.0258.451328
GGAGCAG20600.0244.162635
GAGCAGA20850.0241.235026
CAGAAGC21050.0238.9439
TTAGTAG24500.0238.408199
GGGGAGC22000.0230.636373
GGTTATT25250.0229.574254
GGGGGAG22700.0225.473562
GGGAGCA22550.0224.356984
GCAGAAG23350.0216.038548
AGCAGAA25300.0198.804357
CCAGGTT2150.0157.7907295
GAGGCTT1500.0127.83333295
Produced by FastQC (version 0.11.2)