FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b41.3510000003f73d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b41.3510000003f73d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248450
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA78683.166834373113302No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC60542.436707587039646No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT56372.2688669752465285No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT54582.196820285771785TruSeq Adapter, Index 7 (97% over 38bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA54332.1867578989736365No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA48821.9649828939424432No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA32631.3133427248943448No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG21550.8673777420004025No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA19060.7671563694908432No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC16340.6576776011269874No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15770.6347353592272087No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT13680.550613805594687No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC8850.35620849265445764No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG8630.34735359227208695No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7490.3014691084725297No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7160.28818675789897363No Hit
CCGGGTTATTAGTAGAAACAACAGCTACCCTTATTGAGCATCTCCTGGAT6660.2680619843026766No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT6420.25840209297645406No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5590.22499496880660094No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA5560.2237874823908231No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4720.18997786274904407No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4180.16824310726504327No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA4110.16542563896156168No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT3950.15898571141074663No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT3920.15777822499496882No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA3860.15536325216341315No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3700.1489233246125981No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT3620.14570336083719057No Hit
CCGGGTTATTAGTAGAAACACGAGCGGCGGGGCAGAGAGCAGTGCCACAA3520.14167840611793117No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA3410.13725095592674583No Hit
CCGGGTTATTAGTAGTAACAAGAGCAAAACTCCATCTCAAAAAAACAAAA3300.13282350573556048No Hit
GGGGGGAGCAGAAGCGGAGCGGGGTGTGTGATGTGGGGGTTCTGAGGCCG3150.12678607365667136No Hit
CCGGGTTATTAGTAGAAACACGAGCAGTATTTGCAGCATAGAGGCATTTA3140.1263835781847454No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT3120.12557858724089355No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT3010.1211511370497082No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA3010.1211511370497082No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2800.11269873213926344No Hit
CCGGGTTATTAGTAGAAACACGTGCGGCGGGGCAGAGAGCAGTGCCACAA2760.11108875025155968No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTGCTTCTGCTCCCCCCCCGGGTTA2660.10706379553230028No Hit
GGGGGGAGCAGAAGCGGTGCTGTAGGAAGAGCATGGGCTTTAAAATCCGC2570.1034413362849668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT108.461041E-4295.0295
GGGGGGC108.461041E-4295.02
CCGGGTT29300.0280.904451
GGGGGGA31400.0278.558931
CGGGTTA30000.0274.352
GGGTTAT32300.0254.814243
TATTAGT32550.0252.857137
ATTAGTA32800.0250.92998
GGTTATT33750.0243.866674
TTAGTAG33800.0243.505929
GGGGAGC35800.0243.49863
GTTATTA34750.0236.848925
GGAGCAG37900.0228.839075
GAGCAGA37900.0228.839076
TTATTAG36400.0226.112646
CAGAAGC38800.0222.77069
GCTCGCA403.45608E-11221.25295
GGGGGAG39950.0218.573232
TTCGTAC150.0028483577196.66667295
GCCGTAC150.0028483577196.66667295
Produced by FastQC (version 0.11.2)