FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b4.3510000003f480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b4.3510000003f480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189101
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAATTCAG25571.352187455380987No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA21451.1343144668722005No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTCGTAA18971.0031676194203099No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGC18500.9783131765564433No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACAAGAGC16770.886827674100084No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA15630.8265424297068762No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACAACAGC15600.8249559759070549No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA13530.7154906637193881No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT12750.6742428649240353No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACAACGCA11840.6261204329961237No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10090.533577294673217No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT9930.5251162077408369No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACAGC9340.49391594967768543No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACAAC9330.49338713174441173No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCAGAGGAT9320.4928583138111379No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACAAC8530.45108169708251145No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACGCA8520.4505528791492377No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCAGAGGATTGGGG7790.41194917002025366No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC7670.40560335482096865No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACACGAGC7270.38445063749001857No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAG6930.366470827758711No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCCAAAATTGAA5910.3125313985647881No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG5840.30882967303187187No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACACGTGC5560.2940227709002068No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTAGTAACAAG5500.2908498633005642No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGC5380.2845040481012792No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCACGCTCTTGTTA5320.2813311405016367No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGC5300.28027350463508915No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT5200.27498532530235165No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT5070.26811069216979283No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAATTCAG5030.2659954204366979No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCACGCTCT4900.259120787304139No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA4790.2533037900381278No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4600.24325624930592646No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTGGGGGGAGCAGAAGCAGAGGAT4560.24114097757283148No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT4530.23955452377301018No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACACG4300.22739171130771388No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATAAGA4290.22686289337444013No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACAACAGC4040.21364244504259627No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGTGC3950.20888308364313252No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGTAACAAGAGC3940.20835426570985877No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATGCCTTTATT3860.2041237222436687No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACG3630.1919609097783724No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTCGTAA3490.18455745871253987No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGTAACAAGAGC3430.18138455111289734No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA3390.17926927937980233No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACAACGCA3380.17874046144652858No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACAAGAGG3100.16393355931486348No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT2930.15494365444920968No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACAACGCA2920.15441483651593593No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGG2690.1422520240506396No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT2660.14066557025081836No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCAGGCTCT2590.13696384471790207No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTGTTCATTG2550.13484857298480707No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCAGGCACT2430.12850275778552203No Hit
GGGGGGAGCAGAAGCGGAGCGGGGGGAGCAGAAGCAGAGGATTGGGGGGA2420.12797393985224825No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACAAGTGC2390.126387486052427No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTGTTCATTG2390.126387486052427No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACAACAGC2380.12585866811915325No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCGGTGCGTTGTTT2260.11951285291986823No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAAA2260.11951285291986823No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC2160.11422467358713068No Hit
GGGGGGAGCAGAAGCGGTGCGTTGTTTCTACTAATAACCCGGGCTCTTGT2140.11316703772058319No Hit
GGGGGGAGCAGAAGCAGAGCGGGGGGAGCAGAAGCAGAGGATTGGGGGGA2130.11263821978730942No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT2030.1073500404545719No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATGTTATTAGTAGTAACA2030.1073500404545719No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCAGGCTCTTGTTA1940.10259067905510813No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT1900.1004754073220131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT251.8780338E-7236.02
ATAAGTT301.14757175E-4147.49998295
CCGGGTT108700.0117.782891
CGGGTTA110500.0115.730772
AGAGCTA403.6073115E-4110.62499295
GGGTTAT116050.0110.196043
GGTTATT117300.0108.518764
TTATTAG116850.0108.4317556
GTTATTA117300.0108.015785
CCGGTTA559.516383E-6107.2727361
TATTAGT118450.0107.216137
ATTAGTA119350.0106.284048
GGGGGGA60900.0105.114941
TTAGTAG121400.0104.4892969
CCGGGTA601.4661919E-598.333331
GGTTATA455.7625974E-498.333334
GGGGAGC66800.095.389233
GGGAGCA69000.092.3478244
GGGGGAG69650.091.4862
GGAGCAG69250.091.375455
Produced by FastQC (version 0.11.2)