FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b39.3510000003f720.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b39.3510000003f720.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185928
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT30681.6501011144098792No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA26481.4242072199991396No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT23181.246719160104987No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT22541.212297233337636No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC19501.0487930811927197No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG17420.9369218191988297No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA15580.8379587797426961No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA14960.804612538186825No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT14250.7664257131792952No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT13490.7255496751430661No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12570.6760681554149993No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA12010.6459489694935674No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11650.6265866356869326No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC11480.617443311389355No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11180.6013080332171594No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT10410.5598941525751905No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTACTTTCT9860.5303128092594983No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT9170.4932016694634482No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG9160.4926638268577084No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA8760.4711501226281141No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA8670.4663095391764554No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG7230.3888602039499161No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5890.31678929478077533No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA5680.3054946000602384No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5550.2985026461856203No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG5530.2974269609741405No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG5460.2936620627339615No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT5430.292048534916742No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5160.2775267845617658No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4380.23557506131405706No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3440.18501785637451057No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA3400.18286648595155114No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2930.15758788348177788No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2830.15220945742437933No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT2590.13930123488662277No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG2570.13822554967514306No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2370.12746869756034596No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT2360.12693085495460607No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT2320.12477948453164665No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT2270.12209027150294738No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT2220.11940105847424809No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT2180.11724968805128866No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT2020.10864420635945096No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2020.10864420635945096No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT1970.10595499333075169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT27400.0291.231751
CGGGTTA27600.0288.586942
GGGGGGA22400.0273.92861
TATTAGT29850.0266.834177
ATTAGTA29950.0265.943248
GTTATTA30700.0259.446265
TTATTAG31150.0255.698246
GGGGAGC24300.0253.11733
TTAGTAG32450.0245.454549
GAGCAGA25700.0239.32886
GGAGCAG25750.0238.864075
CAGAAGC25900.0238.050199
GGGAGCA26750.0229.934594
GGGTTAT34850.0228.550953
GGGGGAG27900.0220.456992
GGTTATT36450.0218.518524
GCAGAAG30150.0205.961858
ACCGACC150.0028477837196.66667295
CCACGTT150.0028477837196.66667295
GCCACCC150.0028477837196.66667295
Produced by FastQC (version 0.11.2)