FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b37.3510000003f713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b37.3510000003f713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185803
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA38662.08069837408438No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC33941.8266658773001512No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA32251.7357093265447814No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA32251.7357093265447814No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA28191.5171983229549577No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT27511.4806004208758738No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT22931.2341027862843978No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14010.7540244237175934No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC13600.7319580415816752No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG11260.60601820207424No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10580.5694202999951561No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC9690.5215201046269436No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA8010.4311017583139131No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7860.4230286916788211No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCACAATGGATACTTTTATT6680.359520567482764No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT5420.2917068077479911No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4580.24649763459147594No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT4060.21851100358982362No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3970.21366716360876845No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG3750.20182666587730014No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT3740.20128846143496068No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3680.19805923478092385No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT3330.17922207929904252No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT3120.16791978600991372No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCA2740.147468017201014No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT2500.13455111058486677No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2490.1340129061425273No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA2470.13293649725784837No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA2300.12378702173807743No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTTATATTTATTTTGACAAGA2270.12217240841105902No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG2250.1210959995263801No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT2240.12055779508404062No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTGGCTGATGATTACTTTTGGAAA2070.11140831956426968No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA2030.10925550179491181No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1970.10602627514087501No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1880.10118243515981981No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA1860.10010602627514087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA18850.0286.392581
CCGGGTT24850.0279.56741
CGGGTTA25350.0273.47142
GGGTTAT26550.0261.11113
TATTAGT26900.0258.262057
ATTAGTA27350.0252.934198
GGGGAGC21300.0252.758213
GGTTATT27500.0252.090884
GTTATTA27700.0250.270775
TTAGTAG27750.0248.225239
GGAGCAG22000.0244.715915
TTATTAG29150.0237.821616
GAGCAGA22900.0235.098276
GGGGGAG23400.0230.705122
CAGAAGC23650.0225.771679
GGGAGCA27050.0199.574864
GCTCGCA150.002847782196.66667295
GCAGAAG31250.0171.8088
CGTCTGT200.006733183147.5295
AGCAGAA37500.0143.566677
Produced by FastQC (version 0.11.2)