FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b32.3510000003f6de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b32.3510000003f6de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132458
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA28812.175029065817089No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA23511.7749022331606998No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA22541.7016714732217004No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC21211.6012622869135877No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA20061.5144423137900316No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT16261.227558924338281No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG10310.7783599329598817No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTGTTTGCTGACAT9750.73608238083015No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9150.6907850035482945No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA9010.6802156155158616No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT7550.5699919974633469No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6820.5148801884370895No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6580.4967612375243473No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA6210.4688278548672032No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5880.44391429736218274No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT5860.44240438478612093No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4450.3359555481737607No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT4110.31028703438070937No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT3650.27555904513128693No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAACAAAGAAAACAAAAAAGAAT3570.2695193948270395No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT3550.26800948225097765No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT3330.25140044391429733No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT3030.22875175527336966No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2890.21818236724093676No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2640.19930846004016367No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG2230.16835525223089584No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA2090.1577858641984629No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2060.15552099533437014No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2010.15174621389421553TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA1840.13891195699768985No Hit
GGGGGGAGCAGAAGCAGAGGATTCTGATTCTTGAATACCTTTTTTTCCTT1720.12985248154131876No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1720.12985248154131876No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1620.12230291866100952No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT1510.11399839949266938No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1500.11324344320463844No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA1420.10720379290039107No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG1360.10267405517220554No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG1360.10267405517220554No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT1330.10040918630811276No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCC108.456588E-4295.0295
TACCGCA108.456588E-4295.0295
CCGGGTT14500.0292.96551
GGGGGGA14250.0281.543881
CGGGTTA15250.0279.524572
GGGGAGC15650.0255.415343
TATTAGT17150.0247.69687
ATTAGTA17200.0246.119198
GTTATTA17300.0245.549135
GGAGCAG16400.0244.634175
TTATTAG17500.0242.742866
GGGTTAT17850.0237.98323
GAGCAGA17050.0235.307926
CAGAAGC17350.0229.53899
GGTTATT18700.0227.165774
GGGGGAG17700.0226.666692
TTAGTAG18950.0223.390499
GGGAGCA19550.0204.462924
GCAGAAG20700.0193.816428
AGCAGAA23350.0171.820117
Produced by FastQC (version 0.11.2)