FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b31.3510000003f6c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b31.3510000003f6c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144153
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT25521.7703412346603955No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA21581.4970205268013845No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT21101.4637225725444494No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA20151.3978203714109314No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT16671.1564102030481502No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT16331.1328241521161544No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC16321.1321304447358016No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCACAATGGATACTTTTATT16071.1147877602269811No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA14210.9857581874813566No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT13500.9365049634763064No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT13050.9052881313604296No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA12580.872683884483847No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA12190.8456292966500871No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT11530.7998446095468009No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG11010.7637718257684544No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG10010.6944010877331724No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9720.6742835737029407No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9140.6340485456424771No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA7770.5390106345341408No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7220.5008567286147357No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG6680.4633965300756835No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG6590.45715316365250813No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT6320.43842306438298195No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6120.4245489167759256No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC6040.41899925773310304No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT5930.41136847654922204No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5490.38084535181369794No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5260.3648900820655831No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA4850.3364480794711175No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA4630.32118651710335544No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3750.26014026763230735No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCACAATGGATACTTTTATT3540.24557241264489812No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC3270.22684231337537203No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG3020.20949962886655152No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT2890.20048143292196485No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2760.1914632369773782No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTAT2550.176895381989969No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT2210.15330933105797312No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1860.12902957274562443No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTGATTCTTGGTTCTTTATAT1780.12347991370280188No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG1750.12139879156174342No Hit
GGGGGAGCAGAAGCGGTGCGTTTGATTTGTCACAATGGATACTTTTATTA1720.11931766942068496No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1590.11029947347609831No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCTA1510.10474981443327576No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATTC1490.1033623996725701No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1480.10266869229221728No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCA108.457362E-4295.0295
CCGGGTT22450.0293.685971
CGGGTTA22850.0288.544832
GGGGGGA17550.0279.87181
ATTAGTA23750.0278.23168
TATTAGT23800.0277.647067
TTATTAG24250.0271.88666
TTAGTAG24600.0268.61799
GTTATTA24800.0265.856845
GGGTTAT28050.0235.053473
GGGGAGC21150.0232.234043
GGTTATT28900.0228.140154
GGAGCAG21850.0224.794055
GGGGGAG21900.0224.280822
GAGCAGA22450.0218.78626
CAGAAGC22400.0218.616079
GGGAGCA23950.0205.08354
GAGGCAG150.002847123196.66667295
GCAGAAG26200.0188.034368
AGCAGAA27250.0180.7897
Produced by FastQC (version 0.11.2)