FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b3.3510000003f473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b3.3510000003f473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154380
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTCGTAA29101.8849591916051303No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAATTCAG25971.6822127218551628No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT15210.9852312475709288No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC13670.8854773934447467No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA13280.8602150537634409No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA13110.8492032646715896No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA12370.8012695945070605No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT11440.7410286306516388No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT9600.6218422075398368No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT8200.531156885606944No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATGCCTTTATT7900.511724316621324No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA7860.5091333074232414No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT7770.5033035367275553No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT7640.49488275683378674No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG7460.48322321544241487No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCCAAAATTGAA7110.46055188495919164No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT6240.40419743490089394No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT6120.39642440730664597No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAATTCAG5140.3329446819536209No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTGAAGAAAATGC4990.323228397460811No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT4700.30444358077471173No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTCGTAA4490.2908407824847778No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA4250.2752947272962819No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT3690.23902059852312477No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTGTTCATTG3680.2383728462236041No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTGTTCATTG3650.2364295893250421No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCCAAAATTGAA3220.20857624044565357No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATAAGA3030.19626894675476098No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT2640.1710066070734551No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTGAAGAAAATGCTGGGC2580.16712009327633112No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAGGCAAATAGGCCAA2560.1658245886772898No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC2300.14898302888975257No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAAA2220.14380101049358723No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTGAAGAAAATGCTGGGC2130.13797123979790127No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2000.12955045990413266TruSeq Adapter, Index 3 (97% over 36bp)
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA1700.11011789091851275No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT1640.10623137712138878No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1600.10364036792330612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT18200.0294.189581
GGGGGGA11700.0289.957281
CGGGTTA18850.0284.045072
TTATTAG20900.0256.889956
TATTAGT21000.0256.369057
ATTAGTA21200.0253.950478
GTTATTA21250.0252.658845
CAGAAGC13550.0250.368999
GGAGCAG13600.0249.448535
GGGGAGC13700.0248.704383
GAGCAGA13850.0246.010826
GGGAGCA14350.0236.411154
GGGTTAT22900.0234.454163
GGTTATT23750.0226.063164
TTAGTAG23900.0225.261529
AGCAGAA15200.0224.161187
GCAGAAG15400.0221.249988
GGGGGAG17000.0199.558822
ACGATGG150.0028473176196.66666295
ATCTCGA200.0067320867147.5295
Produced by FastQC (version 0.11.2)