FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b29.3510000003f6b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b29.3510000003f6b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131512
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT28672.1800291988563782No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT21361.6241863860332137No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC20771.57932355982724No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA20561.5633554352454528No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA20191.5352211205061135No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT19261.4645051402153415No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT17611.3390413042155849No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT14691.1170083338402579No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT12630.9603686355617739No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA12040.9155058093558002No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA11790.896496137234625No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT10610.8067704848226778No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG8240.6265587931139364No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA8070.6136322160715372No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA7300.5550824259383174No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTGTTCATTG6830.5193442423505079No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG6150.46763793418091126No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA5970.4539509702536651No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5840.4440659407506539No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT5770.43874323255672487No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT5320.4045258227386094No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTGTTCATTG5100.3877973112719752No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT4110.31251900967212115No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT4110.31251900967212115No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3840.29198856378125193No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3160.24028225561165523No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2610.19846097694506964TruSeq Adapter, Index 3 (97% over 36bp)
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2580.19617981629052864No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT2530.19237788186629357No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT2460.1870551736723645No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTAT2310.17564937039965936No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT2290.17412859662996533No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT2260.1718474359754243No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATTC2100.15968124581787213No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTATGAACTTCAAGCACCAGTAAA1900.14447350812093193No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCTA1850.1406715736966969No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGTGTTCATTG1830.13915079992700286No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC1570.11938074092098058No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA1510.11481841961189854No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1450.11025609830281648No Hit
GGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA1400.10645416387858142No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1390.10569377699373442No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTGTTCATTG1370.1041730032240404No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTGGCTGATGATTACTTTTGGAAA1340.10189184256949935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT18000.0289.26391
CGGGTTA18400.0282.975562
GGGGGGA16850.0281.869451
ATTAGTA19200.0270.41678
TATTAGT19200.0270.41677
GTTATTA19350.0269.08275
TTAGTAG19950.0260.250649
TTATTAG20500.0253.98786
GGGGGAG19650.0241.704832
GGGGAGC20050.0236.147133
GGAGCAG20900.0225.837335
GAGCAGA21650.0218.013876
CAGAAGC22200.0212.612639
GGGTTAT24900.0209.106413
GGTTATT24900.0209.106414
GGGAGCA22750.0208.120864
GCGGCTT150.0028468398196.66667295
GCAGAAG24800.0191.512128
AGCAGAA25800.0184.089147
CCGCCAA1450.0152.58621295
Produced by FastQC (version 0.11.2)