FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b28.3510000003f6a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b28.3510000003f6a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101535
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA38323.774068055350372No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC36213.566257940611612No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA35053.4520116216083125No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT18101.782636529275619No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11661.1483724823952333No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT10421.0262471069089476No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG9400.9257891367508742No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT7520.7406313094006992No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT6990.6884325602009159No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6390.6293396365785197No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4910.4835770916432757No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT4610.4540306298320776No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA4520.44516669128871816No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT4180.411680701236027No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4130.40675629093416066No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA4060.3998621165115478No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3350.32993549022504554No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT3160.3112227310779534No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT2610.25705421775742354No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT2560.25212980745555724No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2540.2501600433348107No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2080.20485546855764022No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1860.1831880632294283No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1680.1654601861427094TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1420.13985325257300438No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT1400.13788348845225784No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1390.13689860639188456No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA1360.13394396021076477No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT1310.1290195499088984No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1240.12212537548628552No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA1230.12114049342591224No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1170.11523120106367263No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG1160.11424631900329935No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT1060.10439749839956665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCA108.4536825E-4294.99997295
CCGGGTT12400.0293.81051
CGGGTTA12800.0285.781222
GGGGGGA13150.0281.539921
GGGTTAT13600.0268.970583
TATTAGT13700.0267.007327
ATTAGTA13750.0264.963658
TTAGTAG13750.0264.963659
GGTTATT14000.0261.28574
GTTATTA14300.0255.80425
GGGGAGC14600.0252.56853
GGAGCAG15650.0234.68055
TTATTAG15700.0232.99366
GGGGGAG15950.0232.115982
GAGCAGA16100.0229.03736
CAGAAGC16250.0225.107689
GTGATCT150.002845887196.666669
GGGAGCA19700.0187.182744
GCAGAAG19800.0185.492428
GCTCGAT255.541671E-5177.0295
Produced by FastQC (version 0.11.2)