FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b25.3510000003f69b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b25.3510000003f69b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155827
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA34612.221052834232835No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC33632.158162577730432No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA32202.066394142221823No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT27971.7949392595634903No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA24221.5542877678451104No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA19121.2270017391081136No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG14990.961964229562271No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT12200.7829195197237963No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11750.7540413407175907No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC9440.6058000218190686No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA7780.49927162815173237No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7600.4877203565492501No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7300.46846823721177977No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT6040.38760933599440406No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5380.34525467345196914No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT4330.27787225577082275No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3850.24706886483087015No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3200.20535593959968423No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG2840.18225339639471977No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT2630.1687769128584905No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2310.14824131889852207No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2150.13797352191853787No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT2050.1315561488060477No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1960.1257805130048066No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1940.12449703838230859No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1880.12064661451481451TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA1860.11936313989231648No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1860.11936313989231648No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA1820.11679619064732044No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA1710.10973708022358128No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG1610.10331970711109115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT17600.0288.295441
CGGGTTA17900.0283.463682
GGGTTAT18100.0280.33153
GGGGGGA15100.0270.57951
GGTTATT19050.0266.351684
TATTAGT19050.0265.577427
ATTAGTA20150.0251.079398
GTTATTA20300.0249.950735
TTAGTAG20250.0249.839529
GGGGAGC16600.0245.240983
GGAGCAG16900.0240.887575
TTATTAG21500.0235.313966
GAGCAGA17450.0233.295126
CAGAAGC17450.0232.449869
GGGGGAG19150.0213.35512
CGAACGA150.0028473428196.66667295
TGATTAA150.0028473428196.66667295
GGGAGCA20750.0196.192784
GCAGAAG23700.0171.14988
AGCAGAA26600.0153.599627
Produced by FastQC (version 0.11.2)