FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b2.3510000003f466.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b2.3510000003f466.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291740
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC82652.8330019880715707No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA78782.700349626379653No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTCGTAA77442.654418317680126No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA72862.497429217796668No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA65112.231781723452389No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAATTCAG40241.3793103448275863No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT37061.2703091794063208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT33941.1633646397477204TruSeq Adapter, Index 19 (97% over 38bp)
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA31481.0790429834784396No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT31241.0708164804277782No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT28400.9734695276616165No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA27810.9532460409954069No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTGAAGAAAATGC23470.8044834441626106No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT21910.7510111743333105No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTCGTAA14190.48639199287036405No Hit
GGGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC12990.44525947761705625No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATAAGA10970.3760197436073216No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA8780.300952903270035No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAATTCAG8620.2954685679029273No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT6640.22759991773496946No Hit
GGGGGGAGCAGAAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC6510.22314389524919448No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAAA5830.19983546993898677No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT5450.186810173442106No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA5010.1717282511825598No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAGGCAAATAGGCCAA4920.1686433125385617No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATGCCTTTATT4790.16418729005278673No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT4730.16213066429012135No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT4490.1539041612394598No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT4180.14327826146568862No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA3640.12476862960170015No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3430.11757043943237129No Hit
CCGGGTTATTAGTAGAAACAACAGCTCCCTGCCCCATACGTGGATGACTC3060.10488791389593474No Hit
GGGGGGAGCAGAAGCAGGCACTTGGACACATTCTGTATTCATCACTGCCA2980.1021457462123809No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT2970.10180297525193667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA305.456968E-12295.02
CCGGTTA108.4617955E-4295.01
CCGGGTT51300.0289.249481
GGGGGGA33050.0283.396361
CGGGTTA52450.0283.188752
GGGTTAT54400.0273.85113
TATTAGT55750.0270.39467
GGTTATT55150.0270.126924
ATTAGTA55900.0269.405188
GTTATTA59150.0252.109045
TTAGTAG60800.0247.450659
GGGGAGC38200.0244.803683
TTATTAG61600.0242.0826
CGGGCTA251.87978E-7236.02
CAGAAGC41500.0224.981939
GGAGCAG43550.0214.052815
GAGCAGA43950.0212.104666
GGGGGAG45650.0204.852132
CGTGGAC150.0028486112196.66667295
GCGAAGC150.0028486112196.666678
Produced by FastQC (version 0.11.2)