FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b19_pb.3510000003f5f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b19_pb.3510000003f5f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263540
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT137475.2162859527965395No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT117954.475601426728391No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA106664.047203460575244No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT103963.944752219776884No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG95623.6282917204219474No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG72442.7487288457160206No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT56062.127191318205965TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT36341.3789178113379372No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA32651.2389011155801777No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA29711.1273430978219625No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT28341.0753585793427942No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT26921.0214768156636564No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTC23700.8992942247856113No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT22730.8624876679062001No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT21020.7976018820672384No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT18910.7175381346285193No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT18850.7152614403885559No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATT16560.628367610229946No Hit
GGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTG15750.5976322379904379No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT12490.47393185095241713No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGTGC11040.41891174015329746No Hit
GAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTCA10540.399939288153601No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACACGAGC10120.38400242847385596No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACACGTGC9800.37186005919405024No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGAGC7640.28989906655536163No Hit
GGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGT7360.2792744934355316No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTA4780.18137664111709798No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAAT4540.17226986415724369No Hit
GCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTCATA4110.15595355543750475No Hit
AGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTCAT3860.14646732943765653No Hit
CGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCTA3840.14570843135766867No Hit
CGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCTA3500.1328071639978751No Hit
GGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATTA2980.11307581391819078No Hit
CGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTGA2960.11231691583820293No Hit
CGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGA2850.10814297639826971No Hit
GAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTT2760.10472793503832437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGC108.4613316E-4295.01
GGGGGGG108.4613316E-4295.01
GGGGGGA40350.0277.087981
CCGGGTT62400.0261.67071
AATTGCG351.2732926E-11252.85713295
CGGGTTA64850.0251.102542
GTTATTA65900.0246.877855
GGGTTAT66900.0243.408083
TTATTAG66950.0242.785646
GGGGGAG46900.0238.70472
ATTAGTA68900.0236.128458
CCGCGAA251.8794344E-7236.0295
GGTTATT69200.0235.744224
TATTAGT69100.0235.4457
TTAGTAG72200.0225.540189
CGGTTAT202.2801305E-5221.252
GGGGAGC53050.0210.754013
GGACTAT150.0028484552196.66667295
GATTGCT150.0028484552196.66667295
GGGAGCA56800.0196.320424
Produced by FastQC (version 0.11.2)