FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b19.3510000003f5ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b19.3510000003f5ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199296
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA54582.738640012845215No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA44212.218308445728966No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC33241.6678709055876686No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA32311.621206647398844No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT29481.4792068079640335No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT28181.4139771997430957No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA26601.334698137443802No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT25241.2664579319203597No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT24061.2072495183044316No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA21331.0702673410404624No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG21321.0697655748233783No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG17070.8565149325626205No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA16800.8429672447013488No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT16700.8379495825305073No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA14930.7491369621066153No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT13820.6934409120102761No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG12540.6292148362235067No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT12240.6141618497109826No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT10980.5509393063583815No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC10290.5163174373795761No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTACTTTCT10120.5077874116891458No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT8950.44908076429030186No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG8660.43452954399486193No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT7980.40040944123314065No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7150.35876284521515733No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA7100.3562540141297367No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT6420.3221339113680154No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA5560.2789820166987797No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG5510.276473185613359No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5240.26292549775208734No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA4900.2458654463712267No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4710.23633188824662812No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4480.22479126525369297No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3430.1721058124598587No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3380.16959698137443802No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT3340.16758991650610147No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT2910.14601396917148363No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT2850.1430033718689788No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA2770.1389892421323057No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG2530.12694685292228644No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT2490.12493978805394991No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTA2490.12493978805394991No Hit
GCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAAAATAGA2250.11289739884393064No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2220.11139210019267823No Hit
AGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAAAATAGACACTA2060.10336384071933204No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT2020.1013567758509955No Hit
GCAGAGGATTATGATGACCTCGATTATTAAAGCAACAAAATAGACACTAT2010.10085500963391138No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTC108.4597856E-4295.0295
CCGGGTT34550.0293.292361
CGGGTTA35050.0289.529242
GGGTTAT35650.0284.656373
GGGGGGA28550.0283.633971
GGTTATT36100.0281.108034
ATTAGTA36250.0279.537938
TATTAGT36350.0278.768927
TTAGTAG36750.0275.734689
GTTATTA37900.0267.757265
TTATTAG38000.0266.66456
GGGGAGC32850.0246.955863
GGAGCAG34050.0238.252585
GGGGGAG34600.0234.891652
GAGCAGA35150.0230.376956
CAGAAGC36000.0224.118069
GGGAGCA37900.0214.439334
GCAGAAG40450.0199.826958
CGGCTAT150.002847937196.66666295
GCGGAGT150.002847937196.666665
Produced by FastQC (version 0.11.2)