FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b18.3510000003f5df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b18.3510000003f5df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186028
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA40742.189992904293977No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA33081.7782269335798913No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA25631.3777495860838154No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAGCAATCAAGTTCAG22461.2073451308405185No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC12360.6644161094028855No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA12020.6461392908594406No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT11610.624099597909992No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT10720.5762573376050917No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT10570.568194035306513No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA10540.5665813748467973No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10420.5601307330079343No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT9950.5348657191390543No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTTTTATATT9910.5327155051927667No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8550.4596082310189864No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7530.40477777538865123No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG6980.3752123336271959No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT6280.3375835895671619No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT6090.32737007332229556No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTTTTATATT5850.31446878964456965No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA5800.31178102221171006No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA4920.2644763153933816No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT4900.2634012084202378No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4650.24996237125593998No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAGCAATCAAGTTCAG4440.2386737480379298No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3990.21448384114219365No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3350.18008041800159114No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3270.17577999010901585No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT3030.16287870643128993No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2730.1467521018341325No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCA2510.1349259251295504No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2500.13438837164297848No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA2400.12901283677725933No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2160.1161115530995334No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAA2090.11234867869353No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT2010.1080482508009547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT19800.0290.53031
CGGGTTA20200.0284.777252
GGGGGGA16850.0267.86351
TATTAGT21650.0265.02317
TTATTAG21600.0264.95376
ATTAGTA22800.0252.302648
GGAGCAG17950.0252.27025
GGGGAGC18050.0250.05543
GTTATTA23100.0247.748925
GAGCAGA18600.0243.45436
GGGTTAT24150.0238.198753
GGGAGCA18950.0238.179414
TTAGTAG24150.0237.587989
CAGAAGC21250.0213.788229
GGGGGAG21350.0212.78692
GGTTATT27350.0210.868364
GCAGAAG22500.0201.91118
AGCAGAA25000.0181.129997
TATCGTT301.1475418E-4147.49998295
CCGCCAA1504.7293724E-1178.66667295
Produced by FastQC (version 0.11.2)