FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b17.3510000003f572.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b17.3510000003f572.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258895
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC48641.8787539349929507No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA43881.6948956140520288No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT43751.6898742733540626TruSeq Adapter, Index 1 (97% over 36bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA42851.6551111454450647No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT30311.1707448965796945No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG30141.1641785279746615No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC21180.8180922767917496No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT20440.789509260511018No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA18970.7327294849263215No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15770.6091272523609957No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA15460.5971532860812299No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC12410.4793449081674038No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT11970.4623496011896715No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT11730.453079433747272No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT9700.37466926746364354No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT9630.37196546862627705No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA9290.35883273141621125No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG8810.34029239653141236No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA7730.29857664304061493No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG7630.29471407327294846No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7550.29162401745881533No Hit
CCGGGTTATTAGTAGTAACAAGAGCGAAACTCCATCTCAAAACAAAATAA6920.2672898279225169No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA6520.2518395488518511No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC6030.23291295699028564No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT5950.2298229011761525No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5600.21630390698932No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT5340.20626122559338728No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT5020.1939010023368547No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG4820.18617586280152185No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA4370.16879429884702293No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT4370.16879429884702293No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4320.1668630139631897No Hit
CCGGGTTATTAGTAGAAACAACAGCAAGGGAGTTAGAATAAAGACCCAAA4080.15759284652079028No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3860.14909519303192414No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG3660.1413700534965913No Hit
GGGGGGAGCAGAAGCGGAGCCCAGGGGGAGTCTCCCCAGTCGATGGGGGA3580.13827999768245813No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA3550.1371212267521582No Hit
CCGGGTTATTAGTAGAAACAACAGCCATTATCTCACAGTTTCTATGGGTC3520.13596245582185829No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA3340.12900983024005871No Hit
CCGGGTTATTAGTAGTAACAAGAGCCAGAGACTGACAACCCAGGGACTGG3120.12051217675119258No Hit
CCGGGTTATTAGTAGAAACAACAGCGACAGCCACATGGAATTTGTAAAAT2770.10699318256436006No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2760.10660692558759341No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2730.1054481546572935No Hit
GGGGGGAGCAGAAGCAGAGGATTGCTTGATCCCAGGAGGTTGAGGCTGCA2630.10158558488962707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT30350.0293.5421
CGGGTTA31500.0282.82542
GGGGGGA26000.0276.846161
GGGTTAT34200.0260.928383
TATTAGT34200.0260.06587
ATTAGTA34250.0258.824838
TTAGTAG36450.0243.607689
TTATTAG36500.0243.273976
GTTATTA36900.0241.036595
GGAGCAG30350.0238.138385
CAGAAGC30750.0235.040669
GAGCAGA30750.0235.040666
GGGGAGC31150.0232.969513
GGTTATT39000.0228.05774
GGGAGCA35500.0204.007034
CGTCGAC150.002848426196.66667295
GGGGGAG37750.0191.847672
GCAGAAG40350.0179.485768
AGCAGAA45300.0159.547457
CCGCCAA950.0155.26317295
Produced by FastQC (version 0.11.2)