FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b15.3510000003f565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b15.3510000003f565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346342
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT59951.7309480224748948No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT56131.6206524187075202TruSeq Adapter, Index 8 (97% over 36bp)
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC54981.587448244798494No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA52631.5195962372452663No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA49461.4280682100351676No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA46931.3550190274353096No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG42031.2135403733881538No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG34550.9975688770059652No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT33850.9773576407135144No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC32730.9450196626455931No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA32070.9259633541412823No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT32030.9248084263531422No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA27220.7859283598293018No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT27020.7801537208886015No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT24210.6990200437717632No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG23350.6741890963267522No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG20990.6060483568264895No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGTATTCATTG20780.5999849859387542No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT16440.47467532092555914No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT16270.46976687782596394No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT16260.4694781458789289No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC15990.4616823833089836No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15460.446379590116128No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA14090.40682331337233135No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC12330.35600649069416934No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC11980.34590087254794394No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG11180.32280231678514304No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA10460.3020136165986222No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT9970.2878657511939066No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT9950.2872882872998366No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT9800.2829573080943114No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9320.2690981746366308No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA8950.2584150925963354No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT6950.20066870318933308No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA6350.1833447863672324No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT5930.1712180445917619No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT5780.16688706538623674No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG5610.16197862228664153No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATT5510.1590913028162914No Hit
GGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTG5000.14436597351750582No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTGGCTCCCTATTTGTGAGTGGAA4840.1397462623649456No Hit
GGGGGGAGCAGAAGCGGTGGAGACGGAGGGTCTGGCCAAATAAGCTGCAG4610.13310542758314037No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG4600.13281669563610535No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATTC4230.12213361359580992No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT3860.1114505315555145No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAAT3850.11116179960847948No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT3710.10711955234998932No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3650.10538716066777924No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTTGATTCTTGGTTCTTTATA3620.10452096482667422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT157.2360017E-6295.02
CCGGGTA157.2360017E-6295.01
GGCTGGG108.4624777E-4295.0295
GCTCGAC108.4624777E-4295.0295
CCGGGTT55250.0290.728521
CGGGTTA56400.0284.80052
GGGGGGA40150.0277.73351
TATTAGT61050.0262.62497
ATTAGTA61250.0261.526558
GTTATTA61500.0261.182955
TTATTAG64050.0250.323966
TTAGTAG64750.0247.389959
GGGTTAT65800.0244.114733
GGGGAGC46800.0237.323723
GGTTATT68000.0236.04
GGGGGAG48350.0230.935882
GGAGCAG50100.0222.280445
GAGCAGA51250.0217.00496
CAGAAGC51950.0213.512979
GGGAGCA54950.0202.929934
Produced by FastQC (version 0.11.2)