FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b14_pb.3510000003f559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b14_pb.3510000003f559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494987
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT118582.3956184707881216No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT117012.363900466072846No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT91611.850755676411704No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT87771.7731778814393104TruSeq Adapter, Index 6 (97% over 36bp)
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG84281.7026709792378387No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA81491.6463058625782092No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG60101.21417330152105No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT56291.1372015830718787No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT49040.9907330899599384No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT41060.8295167347829338No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT36380.7349687971603295No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA25570.5165792232927329No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA22590.456375621986032No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT20530.4147583673914669No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT16860.34061500605066397No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTC13230.2672797467408235No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT12950.2616230325240865No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT12140.2452589663970973No Hit
GGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATTA6270.1266699933533608No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTA5330.10767959562574371No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATT5030.10161883039352548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT45750.0292.420781
CGGGTTA46600.0287.086882
GTTATTA47300.0283.150125
TTATTAG52750.0254.454996
GGGTTAT53150.0251.707433
GGGGGGA40900.0244.510991
GGGGGAG41050.0243.258222
TATTAGT58000.0231.931037
ATTAGTA58500.0229.444448
GGGAGCA47150.0211.786854
GGTTATT65300.0205.55134
GGAGCAG48750.0204.533345
GGGGAGC49150.0203.468983
GCAGAAG49100.0201.873738
CAGAAGC49150.0201.368269
AGCAGAA49550.0200.338047
GAGCAGA49900.0199.228476
GAGGCGT150.002849208196.66667295
TTAGTAG71000.0189.464789
CCGCCAA10400.097.86058295
Produced by FastQC (version 0.11.2)