FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b12b.3510000003f53f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b12b.3510000003f53f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154634
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA53783.4778897267095203No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT48263.120917780048372No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT32352.092036680160896No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC27661.7887398631607538No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA24801.6037870067384923No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA18161.1743859694504442No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA15230.9849062948640014No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA13150.8503951265569021No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG11830.7650322697466275No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG11130.7197640881048153No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA9360.605300257381947No Hit
GGGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC9290.6007734392177658No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9030.5839595431793785No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAACCATTTGTTT8720.5639122055951472No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA7770.5024768162241163No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT7360.47596259554819764No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG6550.4235808425055292No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG6280.40612025815797304No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT6200.40094675168462307No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT5870.3796060374820544No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5520.3569719466611483No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAACCATTTGTTT5230.33821798569525463No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAACCATTTGTTT5200.3362779207677484No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT4980.32205077796603593No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4550.2942431806717798No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT4190.2709624015417049No Hit
GGGGGGAGCAGAAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC4180.27031571323253617No Hit
CCGGGTTATTAGTAGAAACAACAGCGATCCGAAATCCCCACTGCATAGAC3960.2560885704308237No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTGATTCTTGGTTCTTTATA3840.24832831072079878No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTCTGGGTAACAGTGAACACGGGGA3400.21987402511737392No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3020.19529986936896157No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2590.16749227207470543No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2530.16361214221969295No Hit
CCGGGTTATTAGTAGAAACAACAGCCAGCGGAAGACAAGTTTAGTTCCAA2520.16296545391052422No Hit
GGGGGGAGCAGAAGCGGTGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC2450.15843863574634298No Hit
CCGGGTTATTAGTAGAAACAACAGCACTAAGAAGAGAACCTCTTCCTCCA2430.15714525912800548No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT2150.13903798647128057No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2150.13903798647128057No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTTGATTCTTGGTTCTTTATA2080.13451116830709933No Hit
CCGGGTTATTAGTAGAAACAACAGCTCTCGAGATTGGGAATAGGTAATAA2080.13451116830709933No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAACCATTTGTTT2040.13192441507042435No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTGTCAGCCTCTCACACTCTGGG1990.12869097352458062No Hit
CCGGGTTATTAGTAGAAACAACAGCTGCAGGAGGGACTGGGAACACTCCA1990.12869097352458062No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA1960.12675090859707439No Hit
GGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCA1810.11705058395954318No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC1800.11640389565037443No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1790.11575720734120568No Hit
CCGGGTTATTAGTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAA1750.1131704541045307No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA1730.1118770774861932No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG1710.11058370086785571No Hit
CCGGGTTATTAGTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAA1710.11058370086785571No Hit
CCGGGTTATTAGTAGTAACAAGAGCCAGAGACTGACAACCCAGGGACTGG1650.10670357101284322No Hit
CCGGGTTATTAGTAGAAACAACAGCAAGGGAGTTAGAATAAAGACCCAAA1640.10605688270367448No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA1600.10347012946699949No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT108.457957E-4294.999972
CCGGGTA108.457957E-4294.999971
CCGTCGC157.228273E-6294.99997295
CGGGCTA157.228273E-6294.999972
CGGGATA108.457957E-4294.999972
CCGGTTA108.457957E-4294.999971
CCGGGTT38250.0293.071871
CGGGTTA38650.0290.038822
GTTATTA39800.0280.917085
TATTAGT40650.0277.220157
ATTAGTA40800.0275.477948
GGGGGGA18200.0274.7391
TTATTAG41200.0271.729376
GGGTTAT42550.0263.10813
GGTTATT42800.0262.26054
GGGGAGC20150.0247.419343
TTAGTAG45950.0244.602819
GGGGGAG21100.0236.978672
CAGAAGC23100.0214.545469
GGGAGCA24000.0207.729164
Produced by FastQC (version 0.11.2)