FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b12a_xt_half.3510000003f3ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b12a_xt_half.3510000003f3ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473119
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG14550.3075336226192565No Hit
TAATTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT13190.27878821184522284No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTATCACATGTA9680.2045996884504744No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC9540.20164060204726506No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA8670.18325199368446418No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC8300.1754315510474109No Hit
GTCTCAACTGTACAGATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTG8290.17522018773289597No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG8090.1709929214425969No Hit
GGCTTGGGCTGTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAA7850.16592020189423803No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA7680.16232702554748382No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC7250.15323840302334085No Hit
TTCCTATAATGCACGACAGAACAAAAATCAGGCAACTACCCAATCTTCTC7060.14922250004755674No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC6800.14372705387016796No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6540.1382316076927792No Hit
GTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCAAT6530.13802024437826424No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA6410.1354838846040848No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT6370.134638431346025No Hit
TTTTATTACTTTGCTCCTGCCACTCGCGCACCACACCTTTTGAGGCAACA6350.1342157047169951No Hit
GTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAACAGTGGAAGT6340.13400434140248013No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAGTAAGGACAATT6340.13400434140248013No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC6320.13358161477345024No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT6070.1282975319105764No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG6050.1278748052815465No Hit
GATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTGGGGACCACACCTTC5920.12512708219285212No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC5850.12364753899124743No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA5780.12216799578964278No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA5760.12174526916061286No Hit
GCCCAAGCCATTGTTGCAAAAAATCCGATTTTACTAGTAGCGTTAGGGCA5750.12153390584609791No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT5700.12047708927352314No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT5380.11371346320904466No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT5370.1135020998945297No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC5360.11329073658001476No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT5350.1130793732654998No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA5340.11286800995098485No Hit
CATTTGTACAGAAGGGGAAGACCAAATTACTGTTTGGGGGTTCCATTCGG5330.11265664663646989No Hit
GGGATAACATCTTCAAACTCACCTCATGTGGTCAAAACAGCTACTCAAGG5210.11012028686229046No Hit
TCTCAGAGGATATGAAAAGATCAGGTTATCAACCCAAAACGTTATCGATG5180.10948619691874559No Hit
ATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCAAACTCACCTC5120.10821801703165589No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT5070.10716120045908112No Hit
GTCTATATTTGGTTCCATTGGCAAGCTTCAAAGGTGTTTTTACCCATATT5010.1058930205719914No Hit
GTGTTATTATCACTATATGTGTCAGCTTCATTGTCATACTTACTATATTC4960.10483620399941664No Hit
GACACATACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGC4880.10314529748329702No Hit
GTACAAATGTATGGTACTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTA4870.10293393416878206No Hit
GTAATAAAAGGGTCATTGCCTTTAATTGGTGAAGCAGATTGCCTTCATGA4850.10251120753975215No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC4820.1018771175962073No Hit
CCTCTATGGAGACTCAAATCCTCAAAAGTTCACCTCATCTGCTAATGGAG4800.10145439096717739No Hit
CTCCTAATTAGCCCCCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC4740.10018621108008768No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAGTCGGTGCTCTTGACCAAATTG4740.10018621108008768No Hit
GTTACATCCGGGTGCTTTCCTATAATGCACGACAGAACAAAAATCAGGCA4740.10018621108008768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG202.281273E-5221.25295
GCACGCA1150.0218.043472
CGGTGCA900.0196.666672
GGTGCAT950.0186.31583
CACGCAC1350.0185.740743
CGCACTT1350.0185.740745
CCGGGTT255.5544442E-5177.0295
TCGCGCC255.5544442E-5177.0295
AGCACGC1450.0172.931031
GCGGTGC1500.0147.51
GCTCGTT401.9557501E-6147.5295
ACGCACT1750.0143.285724
GCGGAGC1900.0131.97371
ACACGTG802.910383E-11129.06257
ACGTGCA802.910383E-11129.06252
GTAACAA7850.0120.254784
TAGAAAC3000.0103.249992
AAACACG2900.096.637935
GAAACAC3000.093.4166644
CGGTGCG508.7675714E-488.52
Produced by FastQC (version 0.11.2)