FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b12a_xt_full.3510000003f3d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b12a_xt_full.3510000003f3d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548639
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG18610.3392030096292826No Hit
TAATTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT14260.25991590098407147No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTATCACATGTA13170.24004855651894963No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC9840.17935290783192592No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC9470.17260894686670106No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG9270.16896356256117412No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA9130.16641179354730523No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC8500.15492883298489535No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT8420.15347067926268457No Hit
GTCTCAACTGTACAGATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTG8330.15183025632519745No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA7800.14216998791555102No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT7760.14144091105444564No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC7610.13870687282530042No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA7460.13597283459615522No Hit
GGCTTGGGCTGTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAA7450.13579056538087889No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAGTAAGGACAATT7360.13415014244339174No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT7350.13396787322811538No Hit
GCCCAAGCCATTGTTGCAAAAAATCCGATTTTACTAGTAGCGTTAGGGCA7270.13250971950590462No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC7180.13086929656841748No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA6900.12576575854067976No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA6800.12394306638791627No Hit
GTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAACAGTGGAAGT6780.12357852795736358No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT6700.1221203742351528No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6650.12120902815877108No Hit
TTCCTATAATGCACGACAGAACAAAAATCAGGCAACTACCCAATCTTCTC6620.12066222051294202No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC6610.12047995129766567No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC6600.12029768208238933No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT6490.11829272071434951No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG6380.1162877593463097No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT6240.11373599033244082No Hit
GATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTGGGGACCACACCTTC6050.11027287524219022No Hit
GTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCAAT5930.10808564465887405No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA5840.10644522172138693No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC5830.10626295250611058No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAGTCGGTGCTCTTGACCAAATTG5800.10571614486028152No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT5700.10389345270751807No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC5690.1037111834922417No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC5620.10243529898530729No Hit
GTGTTATTATCACTATATGTGTCAGCTTCATTGTCATACTTACTATATTC5590.10188849133947823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA1400.0221.252
CACGCAC1600.0193.593733
CGCACTT1650.0187.727265
CGGTGCA750.0177.02
GCTCGTT255.5549244E-5177.0295
AGCACGC1800.0172.083341
CGGAGCG351.0061012E-6168.571432
GCGGAGC1750.0160.142851
GGAGCGT401.956032E-6147.499983
ACGCACT2200.0140.795474
GGTGCAT1000.0132.753
ACACGTG1103.6379788E-12107.2727361
GCGGTGC1550.0104.677431
GTAACAA10600.0104.3632054
CGTGCCT1001.70985E-10103.254
AAACACG2750.0101.909095
CACGAGC2350.0100.425538
AGCGTTT753.7601058E-798.3333365
GGTCGTC455.768496E-498.333336295
ACGTGCC1052.5102054E-1098.3333363
Produced by FastQC (version 0.11.2)