FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b12a_xt_fourth.3510000003f3fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b12a_xt_fourth.3510000003f3fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282649
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT8630.3053256866289992No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG7020.24836457938998546No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC6210.2197071279219102No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA6170.2182919451333633No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC5200.18397376251110034No Hit
GTCTCAACTGTACAGATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTG4880.17265230020272496No Hit
GGCTTGGGCTGTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAA4850.17159091311131472No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC4610.16309981638003318No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG4610.16309981638003318No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA4520.1599156551058026No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA4460.15779288092298221No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAGTAAGGACAATT4330.1531935368602047No Hit
TTCCTATAATGCACGACAGAACAAAAATCAGGCAACTACCCAATCTTCTC4330.1531935368602047No Hit
GCCCAAGCCATTGTTGCAAAAAATCCGATTTTACTAGTAGCGTTAGGGCA4320.15283974116306798No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT4270.1510707626773843No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTATCACATGTA4260.15071696698024759No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC4190.14824039710029047No Hit
GTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCAAT4170.147532805706017No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAGTCGGTGCTCTTGACCAAATTG4020.14222587024896602No Hit
TTTTATTACTTTGCTCCTGCCACTCGCGCACCACACCTTTTGAGGCAACA3970.14045689176328238No Hit
GTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAACAGTGGAAGT3950.1397493003690089No Hit
GATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTGGGGACCACACCTTC3890.13762652618618854No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA3840.13585754770050487No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA3820.1351499563062314No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC3800.13444236491195793No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT3750.13267338642627427No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC3750.13267338642627427No Hit
GTACAAATGTATGGTACTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTA3730.13196579503200082No Hit
GTCTATATTTGGTTCCATTGGCAAGCTTCAAAGGTGTTTTTACCCATATT3620.12807404236349676No Hit
GGGATAACATCTTCAAACTCACCTCATGTGGTCAAAACAGCTACTCAAGG3550.12559747248353964No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT3500.12382849399785599No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG3380.11958294563221522No Hit
ATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCAAACTCACCTC3370.1192291499350785No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3350.11852155854080502No Hit
ATTTATAGCTTCTTGTGTACTCTTAAGGTCTGCCGCCACTGCCACTCCAT3300.11675258005512136No Hit
ATTAAAGGCAATGACCCTTTTATTACTTTGCTCCTGCCACTCGCGCACCA3210.11356841878089079No Hit
TCTCAGAGGATATGAAAAGATCAGGTTATCAACCCAAAACGTTATCGATG3200.11321462308375406No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCA3150.1114456445980704No Hit
GTTACATCCGGGTGCTTTCCTATAATGCACGACAGAACAAAAATCAGGCA3130.11073805320379693No Hit
GTTTGTGGGTTCGTTGCATTTTTGTAGTTGTCCTTTGGGACAGCCCAAGC3090.10932287041525002No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG3020.1068463005352929No Hit
ATACAGGGGACATCACATTTCTTATCTTTTATTTCGAAGCCAAAGGAATA2990.10578491344388269No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA2980.10543111774674596No Hit
CCTCTATGGAGACTCAAATCCTCAAAAGTTCACCTCATCTGCTAATGGAG2980.10543111774674596No Hit
TTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGG2920.10330834356392557No Hit
CATTTGTACAGAAGGGGAAGACCAAATTACTGTTTGGGGGTTCCATTCGG2880.10189316077537866No Hit
CTCAAGGGGAGGTCAATGTGACTGGCGTGATACCACTGACAACAACACCA2870.1015393650782419No Hit
GTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAGAGTACCACCTT2870.1015393650782419No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT2860.10118556938110519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAGC1050.0182.619051
AGCACGC650.0181.538471
CGCACTT800.0165.93755
GGGGCAG200.0067350273147.5295
GCACGCA800.0147.52
CGGTGCA200.0067350273147.52
ACACGTG453.5119228E-6131.111117
ACGCACT1050.0126.428574
AAGCGGT352.1213705E-4126.4285662
ACGTGCC403.6091785E-4110.6252
CACGTGC804.26553E-9110.6251
CGTGCCT455.7655753E-498.3333363
AGCGGTG455.7655753E-498.3333361
GTAACAA5900.090.000014
CACGCAC1500.088.53
TAGAAAC1550.085.645162
GAAGCGG703.1539123E-584.285711
GCGGTGC703.1539123E-584.285711
ACGTGCA550.001279652880.454552
AACACGT858.2562234E-569.4117666
Produced by FastQC (version 0.11.2)