FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b12a.3510000003f522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b12a.3510000003f522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206740
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA31311.514462610041598No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG30631.4815710554319435No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC23751.1487859146754378No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA23301.1270194447131663No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC18290.8846860791332107No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA14240.6887878494727677No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14210.687336751475283No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTCATT12490.6041404662861566No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12260.5930153816387733No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT12030.5818902969913902No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11600.5610912256941085No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT9960.4817645351649415No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9020.43629679791041887No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA8250.3990519493083099No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG8200.3966334526458354No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7290.3526168133887975No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA7240.3501983167263229No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTATATTCATTG5800.28054561284705426No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5600.2708716261971558No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT5280.2553932475573184No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5270.2549095482248234No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT5190.2510399535648641No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5140.2486214569023895No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4600.22250169294766373No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4130.19976782432040244No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3350.16203927638579857No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3210.15526748573086968No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA3030.1465608977459611No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2960.14317500241849665No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTATATTCATTG2890.1397891070910322No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA2680.12963142110863887No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2630.12721292444616428No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2240.10834865047886234No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTATATTCATTG2230.10786495114636742No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA2170.10496275515139789No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTG108.4600144E-4295.0295
GCTCGCA108.4600144E-4295.0295
CCGGGTT19300.0294.235721
CGGGTTA19700.0288.26142
TATTAGT21650.0264.34187
GGGGGGA16450.0263.6171
ATTAGTA21800.0261.846348
GGAGCAG17800.0243.623615
GGGTTAT23550.0241.13593
GAGCAGA18050.0240.249316
GTTATTA23700.0239.60975
GGGGAGC18100.0239.585653
TTAGTAG24200.0235.87819
CAGAAGC18350.0235.517729
TTATTAG24300.0233.693426
CAAGGTT202.279422E-5221.25295
GGGAGCA20600.0210.509694
GGTTATT27050.0210.480594
CGGGCTA150.0028480128196.666672
GGGGGAG22800.0190.844282
Produced by FastQC (version 0.11.2)