FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_b1.3510000003f45a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_b1.3510000003f45a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259946
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA144705.566540743077408No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC100863.8800366229909287No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA94573.6380632900679375No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA89733.451870773160579No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA62472.40319143206666No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA59892.3039400490871182No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT45841.7634431766597676No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT35181.3533580051241412TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT18530.7128403591515161No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG17130.6589830195502143No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC12760.49087118093757937No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT11500.4423995752964077No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT10390.3996983988982327No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10200.3923891885237703No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA9120.35084209797419463No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT6940.2669785263093104No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT5820.22389265462826896No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5430.20888953859647774No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTTATATTTATTTTAACAAGA4690.18042208766436107No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAGAAACAATCAAGTTCAG4500.1731128772898987No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT4180.16080262823817254No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG4120.1584944565409739No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT3890.14964646503504575No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3840.14772298862071354No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT3700.14233725466058336No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT3550.13656682541758672No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA3430.13195048202318943No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3260.12541066221445993No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT3160.12156370938579551No Hit
GGGGGGAGCAGAAGCAGAGGATTGGAATATGGGACGGAGAAGAGGAGTTC3150.12117901410292906No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA3130.1204096235371962No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA3000.11540858485993245No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT2950.11348510844560024No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2780.10694528863687074No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCA108.4612647E-4295.03
CCGGGTT41550.0291.095031
CGGGTTA42000.0287.6252
GGGGGGA41300.0286.071441
GGGTTAT44250.0272.999973
TATTAGT44550.0271.161627
TTAGTAG44900.0269.047889
GGTTATT45250.0267.292824
GTTATTA45950.0262.89995
ATTAGTA46250.0261.194588
GGGGAGC46900.0251.284653
GGAGCAG47800.0246.553365
CAGAAGC48350.0244.053799
GAGCAGA48550.0242.440786
TTATTAG50700.0238.269246
GGGGGAG50150.0235.294132
GCAGAAG55700.0211.584388
AGCAGAA56200.0209.701977
GGGAGCA59000.0200.254
GTTCGAA150.0028484329196.66667295
Produced by FastQC (version 0.11.2)