FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a97.3510000003f664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a97.3510000003f664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257273
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA130305.064658942057658No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC112054.3552957364356155No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC97803.8014093977992247No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG81123.1530708624690504No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA73392.8526118170192754No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA71002.7597143889953473No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT66182.5723647642776353No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT60572.354308458330256TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG54682.1253687716938816No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT40001.5547686698565337No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA33631.3071717591818808No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG30081.1691860397321134No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT27511.069292152693831No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG25971.0094335589043546No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG25340.9849459523541141No Hit
GGGGGGAGCAAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT24450.9503523494498062No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG18150.7054762839474021No Hit
CGGGTTATTAGTAGAAACAAGGTCTGTATTCGTACTGAAAATCAAGATCA16700.6491159196651028No Hit
GGGGGGAGCGAAAGCAGGCGAGCTACCTAAGAACAGCTAAAAGAGCACAC15820.6149110089282591No Hit
GGGGGGAGCGAAAGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC15290.59431032405266No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAAAGAGGAACAGCTCTTTGGACAC14690.5709887940048121No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14160.550388109129213No Hit
GGGGGGAGCGAAAGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT13910.5406708049426096No Hit
CGGGTTATTAGTAGAAACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC12970.5041337412009811No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA12560.4881973623349516No Hit
GGGGGGAGCGAAAGCAGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAG12310.47848005814834826No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10110.3929677813062389No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9950.38674870662681277No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC9800.38091832411485077No Hit
CGGGTTATTAGTAGAAACAAGGCTGTTAATTGTCAGTTCAGTGTTTTAAT9520.370034943425855No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA9350.36342717657896473No Hit
CGGGTTATTAGTAGAAACAAGGCGCCCGTCACGCACCGCACGTTCGTGGG7670.29812689244499035No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7180.2790809762392478No Hit
GGGGGGAGCGAAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC6670.259257675698577No Hit
GGGGGGAGCAAAAGCAGGCGAGCTACCTAAGAACAGCTAAAAGAGCACAC6220.241766528162691No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6090.23671352998565726No Hit
GGGGGGAGCGAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACC5990.23282660831101593No Hit
GGGGGGAGCAAAAGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT5770.224275380626805No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA5670.22038845895216366No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG5470.21261461560288097No Hit
GGGGGGAGCAAAAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC4740.18424008737799924No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT4710.18307401087560685No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC4470.17374539885646764No Hit
GGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT4450.17296801452153937No Hit
GGGGGGAGCAAAAGCAGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAG3710.1442047941291935No Hit
CGGGTTATTAGTAGAAACAAGGCAGCCACCCGAGATTGAGCAATAACAGG3420.13293272127273364No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3250.12632495442584335No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT3140.1220493405837379No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3110.1208832640813455No Hit
GGGGGGAGCGAAAGCAGGGCGACAAACCTACCGAGCCTGGTGATAGCTGG3060.11893980324402484No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA3020.11738503457416831No Hit
GGGGGGAGCAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACC2980.11583026590431175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG108.4612146E-4295.0295
GGGGGGA57950.0288.891271
GGGTTAT70750.0286.869262
GTTATTA71150.0285.04924
GGTTATT71350.0284.250153
TTATTAG71700.0282.862645
TTAGTAG71900.0282.07588
TATTAGT72150.0281.098426
ATTAGTA72600.0279.15297
TAGTAGA73800.0274.81379
GGGGAGC62500.0266.4443
GGGAGCG46000.0264.538064
GGGGGAG63550.0263.66642
GGAGCGA46200.0263.392855
CGAAAGC46500.0261.37639
GAGCGAA47350.0256.684276
AGCGAAA47450.0256.14337
GCGAAAG47800.0254.267788
CGGGTTA80250.0252.725881
GGGAGCA20150.0223.263054
Produced by FastQC (version 0.11.2)