FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a90.3510000003f658.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a90.3510000003f658.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242847
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC162816.704221176296186No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA77653.1974864832590066No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG64082.6386984397583664No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT58882.4245718497654902No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT47831.9695528460306284TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA46831.9283746556473829No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG39701.6347741582148432No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT33651.3856461063962082No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT29931.2324632381705354No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATGACAA23780.979217367313576No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20760.8548592323561749No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG16650.6856168698810362No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15830.6518507537667749No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA15730.6477329347284505No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG15500.638261950940304No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT11420.47025493417666264No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG11000.45296009421569955No Hit
GGGGGGAGCGAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC10630.4377241637738988No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT9700.39942844671748057No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8970.3693683677377114No Hit
GGGGGGAGCGAAAGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC8520.35083818206525097No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7580.31213068310500025No Hit
GGGGGGAGCAAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC7000.28824733268271796No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA5390.22195044616569282No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4970.20465560620472972No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4820.19847887764724292No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG4750.19559640432041575No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC4490.1848900748207719No Hit
GGGGGGAGCGAAAGCAGGCAGCAGAGGCCATGGAGATTGCTAGTCAGGCC4430.1824193833977772No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA4040.16635988914831148No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG3990.16430097962914922No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCT3740.15400643203333786No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA3280.13506446445704498No Hit
GGGGGGAGCAAAAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC3150.12971129970722306No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3130.12888773589955815No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG2870.11818140639991434No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2600.10706329499643807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC108.4609236E-4294.99997295
CGGGTTA49400.0292.014161
GGGGGGA31750.0289.889771
GGGTTAT50550.0285.079132
GGTTATT50600.0284.505923
GTTATTA50650.0283.642644
TTATTAG51350.0279.776065
TTAGTAG51250.0279.458538
TAGTAGA51800.0276.49139
TATTAGT53600.0267.756536
ATTAGTA53700.0266.983257
GGGGAGC34150.0266.925323
GGGAGCG24050.0265.56134
GGAGCGA24100.0264.398355
CGAAAGC24200.0261.477269
GGTATGC400.0258.125295
GGGGGGG301.5788828E-9245.833341
GCGAAAG25800.0245.261638
GAGCGAA26800.0237.210836
AGCGAAA26800.0237.210837
Produced by FastQC (version 0.11.2)