FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a9.3510000003f430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a9.3510000003f430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236034
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC109674.646364506808341No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT59932.5390409856207157No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG57562.4386317225484464No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA38801.643830973503817No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA35761.51503596939424No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT29511.2502436089715887No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA29191.2366862401179493No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT22940.9718938796952981No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG22180.9396951286679037No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT17900.7583653202504724TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG17580.7448079513968326No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16240.6880364693222163No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG15820.6702424227018141No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA13210.5596651329893151No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT12170.5156036842149859No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10340.43807248108323377No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT9050.3834193378919986No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6570.2783497292762907No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6140.2601320148792123No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5090.21564689832820694No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT4610.19531084504774734No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3500.14828372183668456No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3350.14192870518654094No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA2720.11523763525593769No Hit
GGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATC2630.11142462526585153No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT2590.10972995415914656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA108.460773E-4295.01
CGGGTTA33600.0292.36611
GGGGGGA22200.0291.013521
GGGTTAT34550.0284.327062
GTTATTA35350.0278.309784
GGTTATT35450.0277.524663
TTAGTAG36350.0271.05918
TTATTAG36900.0266.619235
TATTAGT37350.0263.801886
TAGTAGA37400.0263.449229
ATTAGTA37900.0259.584447
GGGAGCG18250.0257.013674
GGGGAGC25000.0254.293
GGAGCGA18600.0252.970435
GGTCAGC351.2732926E-11252.85713295
CGAAAGC18900.0248.955029
GCGAAAG19750.0238.240528
AAACGTT450.0229.44446295
GAGCGAA20600.0228.41026
AGCGAAA20600.0228.41027
Produced by FastQC (version 0.11.2)