FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a89.3510000003f508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a89.3510000003f508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285153
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT118024.138830732974929No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA89703.1456796877465782No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC72882.5558209101780447No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA70132.4593814548680886No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT52221.8312975841039723No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT45981.612467692782471TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG41201.444838385007347No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT37451.3133300368574063No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG33021.1579748415762765No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG27190.9535231963191689No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG24320.8528754738684144No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA21860.7666059974820535No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT21120.7406550167804653No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT17340.6080946018453252No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTATAGGTAGAGGCGACAAACCTA13760.48254796547818185No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA12900.4523887176357955No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA10490.3678726858914337No Hit
GGGGGGAGCGAAAGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT10290.3608589073234369No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8830.30965832377706004No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG7350.2577563623738835No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG5730.20094475597310918No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG5640.1977885556175106No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGGAAGCTTAT3820.13396317064873944No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3310.11607803530034753No Hit
GGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTTC3280.11502596851514801No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3190.11186976815954944No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGG108.4616966E-4294.99997295
GCGTGAG108.4616966E-4294.99997295
GGTCTGG108.4616966E-4294.99997295
CGGGTTA47550.0291.58781
GGGGGGA29950.0289.090151
GGGTTAT48600.0285.288062
GGTTATT48950.0283.24823
GTTATTA49000.0282.658174
TTATTAG50300.0275.646155
TATTAGT50450.0274.826576
ATTAGTA50600.0274.303387
TTAGTAG50900.0272.97648
GGGGAGC31850.0268.60283
GGAGCGA26250.0268.028565
GGGAGCG26250.0268.028564
CGAAAGC26350.0265.332069
TAGTAGA52550.0264.405339
GAGCGAA27400.0256.77926
GCGAAAG27450.0254.699458
AGCGAAA27750.0254.072087
Produced by FastQC (version 0.11.2)