FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a81.3510000003f4fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a81.3510000003f4fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249304
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA169976.8177807014729No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA96993.890430959792061No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA60942.4444052241440173No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC53982.1652279947373487No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA45621.829894426082213No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT35621.4287777171645861No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG29211.1716619067483875No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT21470.8611975740461445TruSeq Adapter, Index 7 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT20690.8299104707505697No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20220.811057985431441No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19700.7901999165677246No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT19000.7621217469434907No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT17630.7071687578217758No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15980.6409845008503674No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15510.622132015531239No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11830.47452106664955235No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG11210.4496518306966595No Hit
GGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAA9970.39991335879087375No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9520.3818631068895806No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA8420.33774026890864167No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA7990.3204922504251837No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7840.3144754997914193No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG6370.2555113435805282No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5120.20537175496582485No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4990.20015723774989572No Hit
GGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAAT4930.19775053749638993No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4890.19614607066071946No Hit
GGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAA4810.19293713698937842No Hit
GGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAAT4330.17368353496133235No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA4180.16766678432756796No Hit
GGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATG3810.15282546609761577No Hit
AAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAAC3130.12554952989121715No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2820.11311491191477073No Hit
GGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATA2770.1111093283701826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA41550.0292.515051
GGGTTAT42350.0287.685942
GGGGGGA35200.0277.400571
GTTATTA44100.0276.26984
TTATTAG44700.0272.561555
GGTTATT44800.0271.953123
TTAGTAG45900.0265.435738
TATTAGT46550.0261.729346
ATTAGTA47050.0259.26147
TAGTAGA47000.0258.909589
GGGGAGC37800.0257.53973
GGGAGCG26150.0248.183554
GGAGCGA26600.0243.984955
GGGGGAG41000.0238.158542
GGGAGCA13650.0237.728934
TGACGCA500.0236.0295
TGACGCT202.2799835E-5221.25295
GAGCGAA29550.0219.627756
CGAAAGC29750.0217.655469
AGCGAAA29850.0217.420437
Produced by FastQC (version 0.11.2)