FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a8.3510000003f423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a8.3510000003f423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200862
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA104955.224980334757197No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC103445.149804343280461No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT58132.894026744730213No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC56632.819348607501668No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG55272.7516404297477868No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT41412.0616144417560314No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG33061.645906144517131No Hit
GGGGGGAGCAAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT22621.126146309406458No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT21561.0733737590982864No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG20811.0360346904840139No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT20651.0280690225129692No Hit
CGGGTTATTAGTAGAAACAAGGGGCAGGGACTTAATCAACGCAAGCTTAT19950.9932192251396481No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA15980.7955710886080991No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG15090.7512620605191624No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT15090.7512620605191624No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT15000.7467813722854497TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGAGCGAAAGCAGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC12330.6138542880186396No Hit
GGGGGGAGCGAAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC10840.539674005038285No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG9820.48889287172287443No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA9660.4809272037518296No Hit
CGGGTTATTAGTAGAAACAAGGGACCGGGGTCCGGTGCGGAGTGCCCTTC8380.41720185998347126No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8110.4037597952823331No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7560.3763778116318667No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA6900.34351943125130685No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTT6640.3305752207983591No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT6270.312154613615318No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6200.30866963387798585No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT5650.2812876502275194No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA5590.27830052473837763No Hit
GGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT5510.27431769075285517No Hit
GGGGGGAGCAAAAGCAGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC5040.25091854108791106No Hit
GGGGGGAGCGAAAGCAGGTTTCAGGTTACAAAGTAAAATCACCATCCGAT4840.2409614561241051No Hit
CGGGTTATTAGTAGAAACAAGGGGGACCGGGGTCCGGTGCGGAGTGCCCT3960.19715028228335874No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3770.18769105156774302No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG3540.17624040385936612No Hit
GGGGGGAGCAAAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC3540.17624040385936612No Hit
CGGGTTATTAGTAGAAACAAGGGCTAATTCAGGCAGAAATTCAAAATGGA3350.16678117314375043No Hit
CGGGTTATTAGTAGAAACAAGGCGGGACCGGGGTCCGGTGCGGAGTGCCC3330.16578546464736985No Hit
CGGGTTATTAGTAGAAACAAGGGCTAATTCTATGGTTTCTGAAAATGTGA3290.16379404765460862No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT3280.16329619340641835No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC2910.14487558622337723No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTT2860.14238631498242574No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2620.13043781302585855No Hit
GGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTCA2580.12844639603309735No Hit
GGGGGGAGCAAAAGCAGGTTTCAGGTTACAAAGTAAAATCACCATCCGAT2520.12545927054395553No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA2370.11799145682110106No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2360.11749360257291075No Hit
GGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATC2300.11450647708376896No Hit
GGGGGGAGCAAAAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC2050.10206012087901147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGC108.459837E-4295.0295
AGGTTAT108.459837E-4295.02
GGGGGGA39050.0282.912931
GGGTTAT48100.0282.120572
TTATTAG49000.0277.23985
GTTATTA49100.0276.675144
TTAGTAG49850.0272.80848
GGTTATT50050.0271.718263
TATTAGT50350.0269.806346
ATTAGTA50400.0269.53877
TAGTAGA50600.0268.76489
GGAGCGA29850.0260.410375
GGGGAGC42750.0258.77193
GGGAGCG30100.0258.737554
GAGCGAA31000.0250.749986
GGGAGCA13250.0247.132084
CGAAAGC31600.0245.988929
AGCGAAA31650.0245.60037
GGGGGAG46450.0238.476852
GCGAAAG33050.0235.196698
Produced by FastQC (version 0.11.2)