FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a78.3510000003f4ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a78.3510000003f4ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285209
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC185276.495938066470554No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA132794.655883930731498No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG82252.883850088882188No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG75702.654193941986403No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT65402.293055268241886No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT44721.5679729601800787No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT43191.514328089225796TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG37931.3299019315659744No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT36211.269595279251356No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA35201.2341826520200976No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA18110.6349729496614763No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA17810.6244543475135778No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17590.6167407059384521No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT17420.6107801647213096No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG16700.5855355195663531No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14930.5234757668937516No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA13240.4642209747939231No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8010.2808466773488915No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5510.19319165944973685No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT5010.17566065586990592No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC4770.1672457741515871No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4760.16689515407999045No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4410.1546234515741088No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT4260.14936415050015955No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA3980.1395467884954542No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC3960.13884554835226098No Hit
GGGGGGAGCGAAAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCC3800.13323562720671508No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT3750.131482526848732No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA3390.11886020427125371No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG3130.10974408240974162No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA3080.10799098205175853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT108.4616954E-4295.01
GGTCCGC157.2346666E-6295.0295
GGGGGGA35250.0291.234041
GGGTTAT56400.0287.154272
CGGGTTA56300.0287.14031
GGTTATT56800.0285.132053
GTTATTA56900.0284.630954
TTATTAG58500.0277.09835
TTAGTAG58450.0276.830638
TATTAGT59500.0272.441166
ATTAGTA59450.0272.42227
GGGGAGC37850.0270.059453
GGAGCGA29750.0269.71435
CGAAAGC29700.0268.678449
GGGAGCG29900.0268.36124
TAGTAGA60500.0267.20669
GAGCGAA30750.0260.463446
AGCGAAA30700.0259.92677
GCGAAAG30850.0258.662878
GGTATGC301.5788828E-9245.83334295
Produced by FastQC (version 0.11.2)