FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a39_xt_half.3510000003f3b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a39_xt_half.3510000003f3b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514544
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA12500.2429335489287602No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG10940.21261544202245097No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA9800.19045990236014804No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG9590.18637861873814485No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT9390.18249168195528467No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT8800.1710252184458472No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA8260.16053048913212475No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG8160.15858702074069467No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC7890.15333965608383346No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA7710.14984141297925932No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA7560.1469262103921142No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT7550.1467318635529712No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT7510.14595447619639915No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG7230.1405127647003949No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT7030.13662582791753475No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG6840.1329332379738176No Hit
GTCCCAGATATGTTAAGCATAGCACTCTGAAATTGGCAACAGGAATGCGA6710.13040672906495848No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA6640.12904630119095745No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA6360.1236045896949532No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA6350.12341024285581019No Hit
TCCCTGTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA6280.12204981498180913No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA6260.12166112130352312No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG6240.12127242762523711No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT6080.11816287819894898No Hit
GTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT6030.11719114400323392No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG5920.11505332877266083No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT5680.11038900463322865No Hit
GTCTAAGAATGTCCACCATCATTGTTCCGCCAATTTGCGTGCTGTGGCAC5620.1092229235983706No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC5490.10669641468951149No Hit
ATATAAGGTTCTTTCCATCCAAAGAATGTTTTTATGCACTTGATCGCATC5490.10669641468951149No Hit
GTGTTAATGTATACCCCCTTCATTATGTATTCAGTAGCTCTACAATGGGA5450.10591902733293945No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5310.10319817158493734No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG5280.10261513106750832No Hit
CTATTAGAAGGGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG5260.10222643738922231No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG5210.10125470319350725No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGGG108.46367E-4295.0295
CGAAAGC2350.0156.91493
CCGAGTG301.1487116E-4147.5295
GCAGGGT2150.0144.069769
GCAGGGG1750.0109.571439
GCAATCC559.532074E-6107.272736295
CTGGCGT455.7682913E-498.33333295
AGCGAAA3750.094.41
AAGTAGG508.7680126E-488.51
GTATTAA1950.083.205131
AGGGTTA1800.081.944449
GGTCGTT555.0245944E-780.454557
AGGGTGT1301.364242E-979.423086
AGCAGGT11250.074.733338
GCCCACC600.001808316473.75295
CTGCGTT600.001808316473.75295
CTACGAA600.001808316473.75295
CAAGGGG600.001808316473.75295
CAAAAGC19950.072.456154
AAGCAGG21200.072.358497
Produced by FastQC (version 0.11.2)