FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a39_xt_full.3510000003f3ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a39_xt_full.3510000003f3ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613766
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA17310.2820293075862788No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG12830.2090373204120137No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT12630.2057787495560197No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG11190.1823170393928631No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA10710.17449646933847754No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT10640.17335596953887963No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG10050.1637431855136974No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA9630.15690018671611006No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG9030.14712447414812813No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC8600.14011854680774108No Hit
GTCCCAGATATGTTAAGCATAGCACTCTGAAATTGGCAACAGGAATGCGA8330.1357194761521492No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA8210.1337643336385528No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT8040.13099454841095792No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG7820.12741012046936454No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT7740.12610669212696696No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA7720.12578083504136756No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA7670.12496619232736905No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA7460.12154469292857538No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT7430.12105590730017628No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG7350.1197524789577787No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA7260.1182861220725814No Hit
TCCCTGTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA7260.1182861220725814No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTC7160.11665683664458441No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT7140.11633097955898503No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT7080.11535340830218684No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC7060.11502755121658742No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA6970.11356119433139013No Hit
CGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATG6870.11193190890339313No Hit
GTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT6530.1063923384482034No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG6380.10394841030620791No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCT6240.10166741070701212No Hit
GTCTAAGAATGTCCACCATCATTGTTCCGCCAATTTGCGTGCTGTGGCAC6240.10166741070701212No Hit
GTGTTAATGTATACCCCCTTCATTATGTATTCAGTAGCTCTACAATGGGA6240.10166741070701212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGT1100.0160.909096
GGCCGCT200.0067369365147.5295
GCAGGGT2900.0137.327599
GCAGGGG3200.0133.671889
CGAAAGC2600.0130.480773
TTGCGTT505.9363083E-6118.0295
GCAGGTT601.4688323E-598.333336295
CCCTGCC857.922972E-786.76471295
GGTCGTT857.922972E-786.764717
AGGTCGT857.922972E-786.764716
TACGGAT2150.082.3255842
AAGCAGG27400.079.133217
GCTATAA4500.078.666671
CGGATAA2300.076.956524
TAACTAT4850.072.989695
AGCGAAA4450.072.921351
CAAAAGC28400.072.7112664
GCAAAAG28350.071.2786563
AGCAGGT14350.070.923348
CTATAAC5000.070.7999952
Produced by FastQC (version 0.11.2)