FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n01_a39_xt_fourth.3510000003f3c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n01_a39_xt_fourth.3510000003f3c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239367
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5960.24899004457590226No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG4890.2042888117409668No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4600.19217352433710577No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4150.17337394043456283No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4020.16794294952938374No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3990.16668964393588087No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA3800.1587520418436961No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3730.15582766212552274No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT3700.15457435653201987No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC3390.14162353206582362No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3290.1374458467541474No Hit
GTCCCAGATATGTTAAGCATAGCACTCTGAAATTGGCAACAGGAATGCGA3290.1374458467541474No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA3240.13535700409830928No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3210.1341036985048064No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3170.13243262438013592No Hit
TCCCTGTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA3070.12825493906845972No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT2980.12449502228795113No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2980.12449502228795113No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2970.12407725375678351No Hit
GTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT2930.12240617963211303No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2810.11739295725810157No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG2810.11739295725810157No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2810.11739295725810157No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA2730.11405080900876062No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG2690.11237973488409012No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2630.1098731236970844No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2620.10945535516591676No Hit
GTCTAAGAATGTCCACCATCATTGTTCCGCCAATTTGCGTGCTGTGGCAC2550.10653097544774343No Hit
GTGTTAATGTATACCCCCTTCATTATGTATTCAGTAGCTCTACAATGGGA2550.10653097544774343No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2540.1061132069165758No Hit
CTATTAGAAGGGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG2530.1056954383854082No Hit
ATATAAGGTTCTTTCCATCCAAAGAATGTTTTTATGCACTTGATCGCATC2530.1056954383854082No Hit
CGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATG2450.1023532901360672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAG200.0067343884147.49998295
GCTAGTT200.0067343884147.49998295
GCAGGGG751.6370905E-11137.666669
GCAGGGT1001.8189894E-12118.07
CGAAAGC1153.6379788E-12102.6086963
GGTAGTT508.762239E-488.57
AGCGAAA1402.5465852E-1184.285711
AGGGTTA1259.968062E-1082.69
TCCAACT1701.8189894E-1278.0882342
ACAAGGT951.5324404E-677.6315843
TCTATGC1751.8189894E-1275.857158
TCGTTGT600.001807130873.74999295
AGGGTGT400.00558810173.749996
GTCGTAC806.112587E-573.749991
CGGATAA901.09531684E-465.555564
AAGCAGG8450.062.8402377
TCGTACT951.4312699E-462.1052672
TACGGAT1001.8445053E-459.02
TAGTTCG150.004380549358.999996290-294
ATTGTCT1605.2029463E-755.3124968
Produced by FastQC (version 0.11.2)